一起急性腹泻疫情中检出的GI.8型诺如病毒的序列测定和分子特征分析

发布时间：2018-01-25 01:31

  本文关键词： 诺如病毒 GI. 全基因组　出处：《中国人兽共患病学报》2017年07期 　论文类型：期刊论文

【摘要】：目的对湖州地区一起急性腹泻疫情中检出的GI.8型诺如病毒进行序列测定和分子特征分析。方法根据GI型诺如病毒保守序列自行设计5对引物,用一步法RT-PCR分段扩增GI.8型诺如病毒CHN/Huzhou/N10的全基因组序列,采用生物信息学相关软件对序列进行整理和分析。结果 CHN/Huzhou/N10全长7 740bp,编码区包括3个开放读码框架(ORF1~ORF3),分别长5 400bp(5~5 404nt)、1 632bp(5 388~7 019nt)和642bp(7 019~7 660nt)。RdRp区,VP1区和VP2区的系统进化分析显示CHN/Huzhou/N10属于GI.8基因型。衣壳蛋白区的氨基酸序列分析表明,与原型株Boxer/2001/US相比,CHN/Huzhou/N10VP1区的氨基酸序列共有16个变异位点,12个位于P2区。其中aa347T→S,aa397T→E这2个变异位点分别位于HBGA受体结合袋位点Ⅱ和Ⅲ内。结论本文获得了我国GI.8型诺如病毒CHN/Huzhou/N10的全基因组序列,相关序列信息可用于病毒的遗传进化研究、快速诊断试剂的研发以及疫苗的设计。
[Abstract]:Objective to determine the sequence and molecular characteristics of GI.8 type Norovirus detected in a case of acute diarrhea in Huzhou area. Methods five pairs of primers were designed according to the conserved sequence of GI type Norovirus. The whole genome sequence of GI.8 type Norovirus CHN/Huzhou/N10 was amplified by one-step RT-PCR. The sequence was sequenced and analyzed by bioinformatics software. Results the length of CHN/Huzhou/N10 was 7 740 BP. The coding region consists of three open reading frames ORF1 / ORF3 with a length of 5 400 BP / 5 404nt respectively. The region of 1 632bpmPU (5 388n) and 642 BP (7 019nt) and 7660nt (RdRp). Phylogenetic analysis of VP1 region and VP2 region showed that CHN/Huzhou/N10 belonged to GI.8 genotype. Amino acid sequence analysis of capsid protein region showed that CHN/Huzhou/N10 belonged to GI.8 genotype. Compared with the prototype strain Boxer/2001/US, the amino acid sequence of CHN / Huzhou-N10VP1 region had 16 mutation sites. 12 are located in P2 region, of which aa347T. 鈫扴,aa397T. 鈫扙. these two mutation sites are located in the HBGA receptor binding pouch site 鈪，

本文编号：1461611