深圳地区四家医院感染性腹泻来源沙门菌耐药性及分子分型研究

发布时间：2018-01-26 05:18

本文关键词： 沙门菌耐药特征脉冲场凝胶电泳　出处：《中国人兽共患病学报》2017年10期 　论文类型：期刊论文

【摘要】：目的分析2012-2016年深圳地区感染性腹泻沙门菌耐药状况及分子型别特征,为沙门菌感染防控提供科学依据。方法收集深圳地区四家医院的137株沙门菌,传统血清学方法鉴定沙门菌血清型,微量肉汤稀释法测定菌株药物敏感性,脉冲场凝胶电泳(PFGE)对鼠伤寒沙门菌和肠炎沙门菌作分子同源性分析。结果 137株沙门菌可分为30种菌型,鼠伤寒沙门菌和肠炎沙门菌是本地优势菌型。菌株对链霉素、氨苄西林、阿莫西林/克拉维酸钾耐药最为严重,对青霉素类、头孢类、氨基糖苷类、大环内酯类抗生素的耐药呈逐年增高的趋势。49株鼠伤寒沙门菌经脉冲场凝胶电泳可获得38种带型,相似度65.5%~100%,TY18为优势带型;27株肠炎沙门菌经脉冲场凝胶电泳可获得9种带型,相似度61.8%~100%,EN7为优势带型。结论感染性腹泻来源沙门菌耐药状况比较严重,菌株PFGE带型多样化,呈高度散发,部分带型与其耐药谱之间具有一定的聚集与关联性。
[Abstract]:Objective to analyze the drug resistance and molecular type of Salmonella infection in Shenzhen area from 2012 to 2016. Methods 137 strains of Salmonella were collected from four hospitals in Shenzhen area. Traditional serological methods were used to identify the serotype of Salmonella. The molecular homology of Salmonella typhimurium and Salmonella enteritidis was analyzed by pulsed field gel electrophoresis (PFGE). Salmonella typhimurium and Salmonella enteritidis are local dominant strains. The strains are most resistant to streptomycin, ampicillin, amoxicillin / clavulanate potassium, penicillin, cephalosporins, aminoglycosides. The resistance of macrolides to Salmonella typhimurium was increasing year by year. Thirty-eight bands of Salmonella typhimurium strains could be obtained by pulsed field gel electrophoresis, and the similarity of 65.5% was 65.5% and TY18 was the dominant band type. Among the 27 strains of Salmonella enteritidis, 9 bands were obtained by pulsed field gel electrophoresis with 61.8% similarity and 100% EN7 as dominant bands. Conclusion the drug resistance of Salmonella enteritidis from infectious diarrhea is serious. The PFGE bands were diversified and highly distributed, and some of the bands had a certain aggregation and correlation with their drug resistance spectrum.
【作者单位】：深圳市罗湖区疾病预防控制中心;深圳市第五人民医院检验科;深圳市人民医院检验科;深圳市远东妇幼保健院检验科;深圳市罗湖区妇幼保健院检验科;
【基金】：深圳罗湖区软科学研究计划项目(No.2015035)资助~~
【分类号】：R446.5;R516.3
【正文快照】： (1.Luohu District Center for Disease Control and Prevention,Shenzhen518020,China;2.Department of Laboratory Medicine,the Fifth People’s Hospital of Shenzhen,Shenzhen518020,China;3.Department of Laboratory Medicine,Shenzhen People’s Hospital,Shenzhen518

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