2016年盐城地区手足口病A组肠道病毒VP1基因特征

发布时间：2018-01-27 07:26

本文关键词： 手足口病基因型 VP基因序列分析　出处：《现代预防医学》2017年15期 　论文类型：期刊论文

【摘要】：目的了解2016年盐城地区手足口病肠道病毒病原谱分布特征,并对鉴定为A组不同基因型肠道病毒VP1基因进行分子进化特征分析。方法通过实时荧光RT-PCR方法对2016年盐城市疑似手足口病咽肛拭子标本进行非EV71非CVA16其他肠道病毒阳性标本的筛选,对分子分型鉴定为A组不同基因型的肠道病毒,用RT-PCR扩增各自VP1基因全长编码区并测序,采用相应的生物信息软件进行核苷酸及氨基酸序列比对及基因种系进化特征分析。结果非EV71非CVA16肠道病毒基因定型结果显示共有7个基因型CVA4、CVA6、CVA10、ECHO3、ECH09、ECHO13、CVB4。遗传进化树显示,盐城地区EV71和CVA16代表株分属于C4a、B1b进化分支,CVA6型代表株分属D1、D3进化分支,CVA10型代表株属于D1基因亚群的毒株,远离各自原型株。CVA4型代表株与近年国内代表株亲缘关系较远,与原型株在同一进化谱系。结论 2016年盐城市手足病流行的病原谱呈现多样性,有多种基因型的肠道病毒同时流行,CVA6是优势病原体,EV71和CVA16仍占有一定比例。
[Abstract]:Objective to investigate the distribution of enterovirus spectrum of hand, foot and mouth disease in Yancheng area in 2016. The molecular evolution characteristics of enterovirus VP1 gene identified as different genotypes of group A were analyzed by real-time fluorescence RT-PCR method. In 2016, the pharynx and anal swabs of suspected hand, foot and mouth disease in Yancheng city were collected from the swabs of pharynx and anal. Non EV71 non CVA16 positive specimens were screened. The full-length coding region of VP1 gene was amplified by RT-PCR and sequenced. The nucleotide and amino acid sequence alignment and phylogenetic characteristics were analyzed by using the corresponding bioinformatics software. Results the results of non-EV71 non-CVA16 enterovirus genotyping showed that there were 7 genotypes CV. A4. CVA6 / CVA10 / ECHO3 / ECH09 / ECHO13CVB4. genetic evolution tree shows that the representative strains of EV71 and CVA16 belong to C4a in Yancheng area. The representative strain of B1b evolutionary branch CVA6 belongs to the representative strain of D1D3 evolutionary branch CVA10 which belongs to the subgroup of D1 gene. The relationship between the representative strain of CVA4 and the representative strain in recent years is far away, and it is in the same phylogenetic pedigree with the prototype strain. Conclusion in 2016, the pathogenic spectrum of hand and foot disease in Yancheng City showed diversity. The enteroviruses with multiple genotypes still have a certain proportion of CVA16 and EV71, which are the dominant pathogens at the same time.
【作者单位】：盐城市疾病预防控制中心;盐城市妇幼保健院;
【基金】：江苏省卫计委医学青年科研课题(Q201513) 盐城市医学科技发展计划项目(YK2015032)
【分类号】：R440;R725.1
【正文快照】： 手足口病(hand,foot and mouth disease,HFMD)是一种常见肠道病毒感染的婴幼儿急性传染病[1-3]。根据国际病毒分类委员会(ICTV)分类,感染人的肠道病毒(Human Enterovirus,HEV)分为A、B、C、D四组,与手足口病有关的主要在A组,少部分在B组。目前,A组肠道病毒有17个血清型包括柯

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