鲍曼不动杆菌对替加环素的耐药性及其稳定性研究

发布时间：2018-01-29 13:21

本文关键词： 鲍曼不动杆菌替加环素诱导耐药适应度代价　出处：《四川大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的了解长期抗菌药物压力及常用紫外线消毒对鲍曼不动杆菌耐药性的影响及替加环素(TGC)耐药鲍曼不动杆菌耐药性在群体中存续的能力。方法选取临床分离TGC敏感鲍曼不动杆菌3株,分别采用多步法及紫外线照射体外诱导耐药,微量肉汤稀释法检测诱导前后鲍曼不动杆菌对TGC的最低抑菌浓度(MIC)变化。选取临床分离TGC耐药鲍曼不动杆菌2株,在空白MHA平板中反复传代,测定其MIC值。最后选取TGC敏感株及TGC诱导同源耐药株进行TGC耐药株适应度代价研究。结果应用多步法成功诱导出TGC耐药株;紫外线照射虽然对TGC耐药性未发生影响,但其MIC值有上升趋势。TGC耐药鲍曼不动杆菌传代40代后菌株MIC值无明显变化。TGC敏感株及TGC诱导同源耐药株进行相同条件下培养,体外单独培养时耐药株达到对数生长期及平台期的时间均较敏感株长;混合培养时,随着连续传代,耐药株菌落数量较敏感株迅速减少,甚至耐药株被清除。结论 TGC可诱导鲍曼不动杆菌产生获得性耐药。鲍曼不动杆菌对TGC的耐药可能具有遗传稳定性。TGC耐药株较敏感株适应性下降。
[Abstract]:Objective to investigate the effect of long-term antimicrobial pressure and ultraviolet disinfection on drug resistance of Acinetobacter baumannii and TGC of tegacycline. The ability of drug resistance of Acinetobacter baumannii to survive in the population. Methods three strains of Acinetobacter baumannii sensitive to TGC were selected. Multistep method and ultraviolet radiation were used to induce drug resistance in vitro. The minimal inhibitory concentration (MIC) of Acinetobacter baumannii to TGC before and after induction was detected by broth dilution method. Two strains of TGC resistant Acinetobacter baumannii were selected. Repeated passage in blank MHA plate. Finally, TGC sensitive strains and TGC induced homologous drug resistant strains were selected to study the cost of TGC resistance. Results the multistep method was used to induce TGC resistant strains successfully. Ultraviolet irradiation had no effect on TGC resistance. However, the MIC value of Acinetobacter baumannii showed an increasing trend. The MIC value of Acinetobacter baumannii strains did not change significantly after 40 generations. TGC-sensitive strains and TGC induced homologous resistant strains were cultured under the same conditions. The time between logarithmic growth stage and plateau stage of drug-resistant strain was longer than that of sensitive strain in vitro. When mixed culture, with continuous passage, the number of drug-resistant strains was decreased rapidly than that of sensitive strains. Even the resistant strain was cleared. Conclusion. TGC could induce acquired drug resistance of Acinetobacter baumannii, and the resistance of Acinetobacter baumannii to TGC may have genetic stability.
【作者单位】：四川大学华西医院感染性疾病中心;
【基金】：科技部国家科技支撑计划课题(No.2012EP001000)资助
【分类号】：R446.5
【正文快照】： 鲍曼不动杆菌是院内感染的重要条件致病菌,具有强大的获得耐药性和克隆传播的能力,多重耐药(MDR)、泛耐药(XDR)鲍曼不动杆菌呈世界性流行[1],在免疫功能低下患者中可引起严重致死性感染。虽然替加环素(TGC)对不动杆菌感染疗效有限[2],但除多黏菌素外,TGC仍是目前治疗MDR/XDR鲍

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