基于光相位下的血全细胞特征分析

发布时间：2018-01-29 15:45

本文关键词： 血细胞免标记相位显微特征分析细胞分类　出处：《江苏大学》2017年硕士论文　论文类型：学位论文

【摘要】：血液分析为个人健康检测提供了丰富的信息,有助于医疗保健专业人员确定各种器官的生理状态,使他们能够进行多种疾病的诊断。近十几年来,定量相位显微技术凭借其非侵入,免标记,快速高效等优势,在血细胞的形态检测和分类上得到迅猛的发展。尤其是在均质红细胞的分析上,定量相位显微技术取得了突破性进展,不仅实现了红细胞的形态参数,化学成分的测定,同时还能运用该技术对红细胞动力学行为进行分析。然而,对于非均质细胞如白细胞和血小板,鉴于其结构的特殊性,目前运用定量显微显微技术对其进行检测方面尚有待发展。本文以数字全息相位显微技术和基于强度传输方程的定量相位显微技术为主要应用技术基础,分别讨论了强度分析法和相位调制法下的定量相位显微成像技术的原理和特点。重点对三种典型的数字全息技术光路和恢复方法进行分析,并对这三种技术分别从稳定性和采样速度和空间带宽利用率等方面进行讨论。本文基于光强传输方程原理,应用相位显微成像软件Biophase与奥林巴士GX51金相倒置显微镜结合组成的相位显微成像系统,分别对血全细胞中的红细胞,血小板和中性粒细胞进行了相位显微成像实验。根据血细胞光学传输理论和特性分析,提出了均质红细胞、非均质血小板和白细胞相位显微下的特征分析方法:相位梯度法和主要常规分析参数法。应用该方法可对血细胞进行三分群和五分类的识别和检测。针对血小板动力学研究的需要,在对血小板的临床研究中,与医学院课题组合作,基于脓毒症中的血小板活化模型下,对血小板进行相位显微成像以及其他血细胞显微观察方法成像(如扫描电镜法),应用本文所提出的相位特征分析方法分别对三种不同刺激下的血小板进行成像观察和相位特征分析,获得了许多有益的结果,从而得到了外源性CO可抑制血小板α颗粒的释放的结论。由此得到了本文方法的应用意义和价值,我们相信本文的方法通过进一步的研究可在血检应用领域发挥更大的作用。
[Abstract]:Blood analysis provides abundant information for personal health monitoring, help healthcare professionals to determine the physiological state of the various organs, so that they can make diagnosis of many diseases. In recent years, quantitative phase microscopy with its non-invasive, label free, fast and efficient, rapid development in the form of detection and classification of blood cells. Especially in the analysis of homogeneous red blood cells, quantitative phase microscopy technology has made a breakthrough, not only to achieve the morphological parameters of red blood cells, the determination of chemical composition, but also can carry on red blood cell kinetics were analyzed by the technique. However, for the heterogeneous cells such as leukocytes and platelet, in view of the special nature of its structure, the use of quantitative microscopy to detect microscopic aspects remains to be developed. In this paper, a digital holographic microscopy based on phase and intensity Quantitative phase microscopy transmission equation based on the main application of technology, the principle and characteristics of quantitative phase imaging technology strength analysis method and phase modulation method were discussed. The analysis focuses on three typical digital holography and optical path restoration method, and the three were from the stability and sampling rate the speed and spatial bandwidth are discussed. The principle of transport of intensity equation based on the application of phase imaging software Biophase and GX51 combined with metallographic microscope Olympus bus phase imaging system composed of blood cells, respectively in red blood cells, platelets and neutrophils were imaging experiments. According to the analysis of blood cell microscopic phase the optical transmission theory and characteristics, puts forward the homogeneous red blood cells, white blood cells and analysis method of characteristic phase heterogeneous platelet micro phase under the ladder The main routine analysis method and parameter identification and detection method. The method can be applied to the three and five group classification of blood cells. The need for research on platelet dynamics, in clinical research on platelet, and medical research group, based on sepsis in platelet activation model, phase microscope imaging and other blood cell microscopic observation method of platelet imaging (such as scanning electron microscopy), phase feature analysis method proposed in this paper to analyze imaging and phase characteristics of three kinds of stimulation of platelets, obtained many useful results, thus obtained the release of conclusion exogenous CO can inhibit platelet alpha the particles are obtained. The method of application of meaning and value, we believe that this method through further research can play a greater role in the blood test applications.

【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.1;TP391.41

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