昆明市静脉吸毒感染者HIV-1env基因C2-V4区序列特征分析

发布时间：2018-01-30 02:18

本文关键词： 人免疫缺陷病毒型序列分析流行病学研究　出处：《中华疾病控制杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的分析昆明市静脉吸毒(intravenous drug user,IDU)感染者艾滋病病毒1型(human immunodeficiency virus type 1,HIV-1)env基因C2-V4区序列特征及代表性广谱单克隆中和抗体(broadly monoclonal neutralizing antibodies,bm NAbs)的表位变异。方法在昆明市收集165份IDUHIV-1感染者血浆样品,提取RNA,通过逆转录得到cDNA,巢式聚合酶链反应(nested-polymerase chain reaction,nested-PCR)扩增HIV-1膜蛋白基因C2-V4区,应用Bio Edit和MEGA等生物信息软件分析所获得序列的特征,并分析2G12、PGT135、PGT128、b12和VRC01等bm NAbs的表位变异。结果 165条序列主要为CRF08_BC(74.55%)和CRF07_BC(23.03%)重组毒株,各可变环变异程度由小到大为V3区C3区V4区,并且CRF08_BC样本的C2-C4、V3区、C3区和V4区基因离散率分别大于CRF_07BC的相应区域;V3环顶端四肽存在5种类型:GPGQ(95.76%)、GPGR(1.82%)、GPRQ(1.21%)、GPGL(0.61%)和RPGQ(0.61%);辅助受体主要为CCR5(97.58%)。90%以上的CRF08_BC和CRF07_BC重组毒株存在与2G12识别相关的糖基化位点的缺失;而b12、VRC01、PGT135和PGT128等抗体识别的位点在CRF08_BC和CRF07_BC重组毒株中的变异率低。结论 CRF08_BC和CRF07_BC为昆明市IDU感染者的主要流行毒株;CRF08_BC基因离散率高于CRF07_BC毒株;V3区序列变异程度小于C3区及V4区;2G12、PGT135、PGT128、b12和VRC01等代表性bm NAbs表位在CRF08_BC和CRF07_BC毒株存在不同程度的变异。
[Abstract]:Objective to analyze intravenous drug user of intravenous drug abuse in Kunming. Immunodeficiency virus type 1. Sequence characteristics and representative broad-spectrum monoclonal neutralizing antibodies of HIV-1)env gene C2-V4. Broadly monoclonal neutralizing antibodies. Methods 165 plasma samples of IDUHIV-1 infected patients were collected in Kunming, and cDNA was obtained by reverse transcription. Nested polymerase chain reaction. The C2-V4 region of HIV-1 membrane protein gene was amplified by nested-PCR, and the sequence characteristics were analyzed by Bio Edit and MEGA. The PGT135hPGT128 of 2G12 was analyzed. Epitope variation of b12 and VRC01 bm NAbs. Results the 165 sequences were mainly composed of CRF08S, BCn74.55) and CRF07BCm23. 03). Recombinant strain. The variation degree of each variable ring was from small to large to that of V3 region C3 region V4, and the C2-C4 V3 region of CRF08_BC samples. The gene dispersion rates of C3 region and V4 region were higher than those of CRF_07BC. There are five types of peptide at the top of the V3 ring: GPRQ1: GPRQ1. 21. GPGL 0.61) and RPGQ 0.61; More than 90% of the recombinant CRF08_BC and CRF07_BC strains of CCR5O97.58 had the absence of glycosylation sites related to the recognition of 2G12. And b12, VRC01. The mutation rate of antibody recognition sites such as PGT135 and PGT128 in the recombinant strains of CRF08_BC and CRF07_BC is low. CRF08_BC and CRF07_BC were the main infectious strains of IDU infection in Kunming. The dispersion rate of CRF08_BC gene was higher than that of CRF07_BC strain. The variation of V3 region was less than that of C3 region and V4 region. 2G12,PGT135,PGT128. The representative bm NAbs epitopes, such as b12 and VRC01, were mutated to different degrees in CRF08_BC and CRF07_BC strains.
【作者单位】：中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室;
【基金】：国家自然科学基金(81172809)
【分类号】：R512.91;R749.64
【正文快照】： 艾滋病病毒1型(human immunodeficiency virus列进行比对。NCBI中Genotyping在线工具(http://type 1,HIV-1)包膜蛋白通过结合宿主细胞表面的www.ncbi.nlm.nih.gov/projects/genotyping/form-CD4分子及CCR5/CXCR4辅助受体分子启动感染page.cgi)初步判断序列亚型[3,5],MEGA 5.05

【相似文献】