脐血源EPC联合造血干细胞移植在造血重建和GVHD中作用研究

发布时间：2018-01-31 11:27

本文关键词： 造血干细胞联合移植脐血源内皮祖细胞　出处：《基因组学与应用生物学》2017年10期 　论文类型：期刊论文

【摘要】：针对脐血源内皮祖细胞(endothelial progenitor cell,EPC)与造血干细胞(hematopoietic stem cell,HSC)联合移植方式进行研究,并探讨移植后的造血细胞重建及移植物抗宿主病(graft versus host disease,GVHD)的影响。构建人-鼠移植EPC与HSC模型,采用移植HSC的小鼠为参考对比,分析移植后的体内植入及造血重建状况,并分析小鼠有无GVHD相关症状,最后采用病理对GVHD进行实验验证。EPC与HSC联合移植小鼠组(n=10)的实验组,生存的平均时间约54 d,长时间生存率接近70%;单纯移植小鼠组(n=10)的实验组中,生存的平均时间约51 d,长时间生存率达到60%;EPC与HSC联合移植小鼠组与单纯移植小鼠组进行比较,生存时间有显著的增高趋势,但经统计学分析两者比较无显著差异性(p=0.314)。在血细胞恢复实验中,联合移植组相比较单纯组,随着时间的增加,HBG、WBC及PLT的恢复值均有增高趋势。联合移植组小鼠骨髓中人CD45比例为(18.21±10.57)%,单纯组骨髓中人CD45嵌合比例为(13.27±6.36)%,无明显统计学差异性(p=0.308);在联合移植组脾脏中的嵌合比例为(5.14±2.97)%,在单纯移植组比为(4.91±7.56)%,无显著统计学差异(p=0.840)。在联合移植组中检查到2只小鼠带有GVHD病症,表现是轻中度活动度明显减少和体重有下降趋势;单纯移植组检查到3只小鼠带有GVHD病症,表现为活动度下降、体重下降、弓背姿势及耸毛现象;共同点为带有GVHD症状的小鼠均有骨髓与小肠不同损伤情况。本研究初步认为脐血源内皮祖细胞与造血干细胞联合移植的方式,能够对移植后的造血系统重建及移植物抗宿主病产生一定的积极影响,但是效果并不显著。
[Abstract]:The umbilical cord blood derived endothelial progenitor cells (endothelial progenitor cell, EPC) and hematopoietic stem cells (hematopoietic stem cell, HSC) of combined transplantation of hematopoietic cells, and to explore the reconstruction after transplantation and graft-versus-host disease (graft versus host disease, GVHD). The effects of the construction of human mouse transplanted EPC and HSC model the transplantation of HSC mice, for reference, analysis after transplantation in vivo and hematopoietic reconstitution, and analysis of mice without GVHD related symptoms, the pathology of GVHD to validate the.EPC and HSC transplantation group (n=10) mice in the experimental group, the average survival time of about 54 d long time survival the rate of nearly 70%; the transplantation of mice (n=10) in the experimental group, the average survival time of about 51 d long time, the survival rate reached 60%; EPC and HSC transplantation mice compared with transplantation of mice, the survival time was The increasing trend, but statistical analysis compared no significant difference (p=0.314). The blood cell recovery experiments, transplantation group compared with the simple group, with the increase of time, HBG, WBC and PLT value increased. The proportion of CD45 in bone marrow transplantation group was (18.21 + 10.57)%, simple group in bone marrow of human CD45 chimeric ratio (13.27 + 6.36)%, no significant difference (p=0.308); chimeric ratio in combined transplantation group in the spleen was (5.14 + 2.97)% in BMT group than for (4.91 + 7.56)%, and there is no statistically significant difference (p=0.840). In the combined transplantation group detected 2 mice with GVHD disease is mild to moderate activity decreased obviously and weight decreased; simple transplantation group detected 3 mice with GVHD disease, manifested as decreased activity, weight loss, bow back posture and towering hair with common phenomenon; GVH Different injury mice were bone marrow and intestinal D symptoms. In this study, human umbilical cord blood derived endothelial progenitor cells and hematopoietic stem cell transplantation, can have a positive impact on the hematopoiesis reconstruction after transplantation and graft-versus-host disease, but the effect is not significant.

【作者单位】：山东省乐陵市中医院;
【基金】：山东省乐陵市中医院资助
【分类号】：R457.7
【正文快照】： *通讯作者,wangsj627@163.comEffect of CB-EPCs Combined with Hematopoietic Stem Cell Transplantationon Hematopoietic Reconstitution and GVHDWang Shujie*Lingling City Hospital of Shandong Province,Dezhou,646099*Corresponding author,wangsj627@163.com联合造血

【相似文献】