泛耐药肺炎克雷伯菌噬菌体SH-Kp152234的生物学特性和基因组学研究

发布时间：2018-02-16 10:06

本文关键词： 肺炎克雷伯菌噬菌体生物学特性基因组学　出处：《上海交通大学学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的·针对泛耐药肺炎克雷伯菌寻找宽宿主谱噬菌体,并对其生物学特性、基因组成进行分析。方法·以泛耐药肺炎克雷伯菌为宿主菌,从医院污水中分离纯化得到针对宿主菌的泛耐药肺炎克雷伯菌噬菌体。电子显微镜观察噬菌体的大小和形态,分析其效价、宿主谱、酸碱稳定性和热稳定性。抽取噬菌体DNA进行测序及分析。结果·分离到l株肺炎克雷伯菌噬菌体,命名为SH-Kp152234。电子显微镜观察显示其属短尾噬菌体,基因组为线状双链DNA,全长为40 578 bp,GC含量为52.85%。预测共有49个开放阅读框,保守序列具有已知的相关功能。结论·筛选得到肺炎克雷伯菌噬菌体SH-Kp152234的宿主谱较宽,潜伏期较短,可适应不同的温度和p H值,为治疗泛耐药肺炎克雷伯菌感染提供潜在依据。
[Abstract]:Objective: to search for a wide host spectrum phage against pan-resistant Klebsiella pneumoniae, and analyze its biological characteristics and gene composition. Methods: using Pan-resistant Klebsiella pneumoniae as host strain, Pan-drug-resistant Klebsiella pneumoniae phage was isolated and purified from hospital sewage. The size and morphology of phage were observed by electron microscope and its titer and host spectrum were analyzed. Acid and base stability and thermal stability. DNA was extracted for sequencing and analysis. Results: 1 strain of Klebsiella pneumoniae was isolated and named as SH-Kp152234. The genome is a linear double-stranded DNA with a GC content of 40 578 BP ~ (-1) and a GC content of 52.85. It is predicted that there are 49 open reading frames, and the conserved sequences have known related functions. Conclusion: the host spectrum of Klebsiella pneumoniae phage SH-Kp152234 is wider and the incubation period is shorter. It can adapt to different temperature and pH value and provide potential evidence for the treatment of pan-resistant Klebsiella pneumoniae infection.
【作者单位】：上海交通大学基础医学院免疫学与微生物学系;优仕美地医疗;复旦大学附属华山医院抗生素研究所;
【基金】：国家自然科学基金(81471908)~~
【分类号】：R440

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