白色念珠菌菌株的生物膜形成率及其对抗真菌类药物的敏感性

发布时间：2018-02-20 18:28

本文关键词： 抗真菌药物白念珠菌多微生物生物膜复发性外阴阴道念珠菌病　出处：《中国临床药理学杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的研究白色念珠菌菌株的生物膜形成率及其对抗真菌剂的敏感性。方法收集本院165例从外阴阴道假丝酵母菌病(VVC)或复发性外阴阴道假丝酵母菌病(RVVC)患者分离的白色念珠菌菌株,以药敏实验评估白色念珠菌株的抗真菌药物敏感性。选取菌株C54用于体内外实验:体外诱导单生菌或多微生物组合的生物膜,并测定其在游离和生物膜形式下的最低抑菌浓度(MIC)值。小鼠体内注射白色念珠菌或混合微生物悬浮液构建VVC模型,用酶联免疫印迹法检测OD595值来观察各组生物膜生长速率。结果对伊曲康唑耐药的白色念珠菌菌株最多,占总数的4.20%。各组生物膜形式的MIC值均高于其游离形式,真菌和细菌菌株的共培养所形成的多微生物生物膜对各种抗真菌药物具有明显的耐药性,经诱导后在生物膜形式下,C54+大肠杆菌组对两性霉素B、伊曲康唑、5-氟胞嘧啶和氟康唑的MIC值为512,256,512,1024μg·mL~(-1);C54+无乳链球菌组对上述抗真菌药物的MIC为512,256,512,1024μg·mL~(-1)。从注射混合微生物悬浮液的小鼠分离的阴道样品中,生物膜形成速率显著高于注射标准白色念珠菌悬浮液的小鼠,差异有统计学意义(P0.05)。在24,48,72 h,ATCC14053组的OD595值分别为0.20±0.11,0.24±0.024,0.25±0.06;C54+大肠杆菌组的OD595值分别为0.69±0.88,0.79±0.65,1.10±0.64;C54+无乳链球菌组的OD595值分别为0.68±0.42,0.81±0.77,1.10±0.10。结论多微生物生物膜的形成能提高白色念珠菌的生物膜形成速率和抗真菌剂耐药性,这将增强对RVVC患者的阴道损伤。
[Abstract]:Objective to study the biofilm formation rate of Candida albicans and the sensitivity of antifungal agents. Methods 165 patients with vulvovaginal candidiasis or recurrent vulvovaginal candidiasis were collected from our hospital. Candida albicans, The antimicrobial susceptibility of Candida albicans strain C54 was evaluated in vitro and in vivo. The VVC model was established by injecting Candida albicans or mixed microorganism suspensions into mice by measuring the minimum inhibitory concentration (MIC) in the form of free and biofilm. The growth rate of biofilm in each group was observed by enzyme linked immunoblot assay. Results the strain of Candida albicans resistant to itraconazole was the most, accounting for 4.20% of the total. The MIC value of biofilm in each group was higher than its free form. The multi-microbial biofilms formed by co-culture of fungi and bacterial strains have obvious resistance to various antifungal drugs. After induction, the MIC values of Amphotericin B, itraconazole 5-fluorocytosine and fluconazole in Escherichia coli group C54 were 512256U 512C54 1024 渭 g 路mL ~ (-1) 渭 g 路mL ~ (-1). The MIC of non-lactobacillus group was 5122566 (5121212) 1024 渭 g 路mL ~ (-1). In vaginal samples isolated from mice, The rate of biofilm formation was significantly higher than that of mice injected with standard Candida albicans suspension. The OD595 values of ATCC14053 group were 0.20 卤0.110.24 卤0.024U 0.25 卤0.06c54, respectively, and the OD595 values of Escherichia coli group were 0.69 卤0.880.79 卤0.651.10 卤0.64C54, respectively. Conclusion the formation of biofilm of Candida albicans can be enhanced by polymicrobial biofilm formation, which is 0.68 卤0.42c0.81 卤0.771.10 卤0.10.The value of OD595 in the group of non-lactobacillus is 0.68 卤0.42c0.81 卤0.771.10 卤0.10.Conclusion the formation of biofilia albicans biofilms can be enhanced by polymicrobial biofilm formation. Rate of formation and resistance to antifungal agents, This will enhance vaginal injury in patients with RVVC.
【作者单位】：温州医科大学附属第一医院检验科;温州医科大学附属第一医院妇科;温州医科大学第一临床医学院;
【基金】：国家自然科学基金青年科学基金资助项目(81501823) 浙江省医药卫生科技计划基金资助项目(2017KY459;2016KYA137) 浙江省自然科学基金资助项目(LY17H200005) 温州市公益类科技计划基金资助项目(Y20160071;Y20160071;Y20140707)
【分类号】：R446.5

【相似文献】