超声联合微泡对不同凝龄富血小板血栓和富红细胞血栓的溶栓效果

发布时间：2018-02-21 06:19

本文关键词： 血栓凝龄超声检查微泡血小板红细胞溶栓　出处：《中国医学影像技术》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨超声联合微泡对不同凝龄富血小板血栓(PRT)和富红细胞血栓(ERT)的溶栓效果。方法分别于体外和大鼠体内颈总动脉制备不同凝龄的PRT和ERT。离体及在体实验均分为4组,即离体PRT 3h组、离体PRT24h组、离体ERT 3h组、离体ERT 24h组及在体PRT 3h组、在体PRT 24h组、在体ERT 3h组、在体ERT 24h组;分别于体外循环装置和大鼠颈总动脉血栓模型中进行超声联合微泡溶栓实验,并采集超声图像。行病理组织学检查以验证血栓成分。离体实验主要分析溶栓后管腔横截面积增加百分比及溶栓率,在体实验主要分析溶栓后血管再通率及颈总动脉血流速度。结果溶栓治疗后,离体及在体实验显示:PRT 3h组与ERT 3h组管腔横截面积增加百分比[(121.12±13.21)%vs(130.09±15.34)%]、溶栓率[(39.83±7.09)%vs(42.14±5.17)%]、血管再通率(83.33%vs 91.67%)及颈总动脉血流速度[(0.21±0.02)m/s vs(0.22±0.01)m/s]差异均无统计学意义(P均0.05)。PRT 24h组与ERT24h组比较、PRT 24h组与PRT 3h组比较、ERT 24h组与ERT 3h组比较,管腔横截面积增加百分比、溶栓率、血管再通率、颈总动脉血流速度均下降(P均0.05)。与ERT 24h组比较,PRT 24h组管腔横截面积增加百分比、溶栓率、血管再通率及颈总动脉血流速度均下降(P均0.05)。结论超声联合微泡对溶解离体及在体大鼠颈总动脉PRT和ERT的效果随血栓凝龄的增加而下降,且以PRT为著。
[Abstract]:Objective to investigate the thrombolytic effects of ultrasound combined with microbubbles on thrombocytic thrombus (PRT) and erythrocytic thromboembolism (ERT) of platelet-rich thrombus (PRT) and erythrocytic thromboembolism (ERT) at different ages. Methods in vitro and in vivo, PRT and ERT were prepared in the common carotid artery of rats and in vitro, and were divided into 4 groups in vitro and in vivo. That is, in vitro PRT 3 h group, isolated PRT24h group, isolated ERT 3 h group, isolated ERT 24 h group and in vivo PRT 3 h group, in vivo PRT24h group, in vivo ERT 3 h group, in vivo ERT 24 h group; The thrombolytic effects of ultrasound combined with microbubble were studied in cardiopulmonary bypass (CPB) and rat common carotid artery thrombosis model. The ultrasonic images were collected and the thrombus components were verified by histopathological examination. In vitro, the percentage of cross sectional area and thrombolytic rate of the lumen after thrombolysis were analyzed. Results after thrombolytic therapy, the rate of recanalization and the blood flow velocity of common carotid artery were analyzed in vivo. In vitro and in vivo experiments showed that the cross sectional area of the lumen increased by 121.12 卤13.21 vs 130.09 卤15.34%, the thrombolysis rate was 39.83 卤7.09 vs 42.14 卤5.17%, the vascular recanalization rate was 83.33 vs 91.67%, and there was no significant difference in the blood flow velocity of the common carotid artery [0.21 卤0.02 Ms vs(0.22 卤0.01 Ms] between the PRT group and the ERT24h group (P < 0.05). The comparison between 24 h group and 3 h PRT group was compared with that of 24 h group and 3 h group of ERT. The percentage of cross sectional area increased, thrombolysis rate, vascular recanalization rate, carotid artery blood flow velocity were all decreased (P < 0.05), compared with ERT 24h group, the percentage of lumen cross-sectional area increased and thrombolytic rate was increased. Conclusion the effects of ultrasound combined with microbubbles on the dissolution of PRT and ERT in the common carotid artery in vitro and in vivo decreased with the increase of thrombocoagulation age, and PRT was the main factor.
【作者单位】：南方医科大学南方医院心内科;深圳市孙逸仙心血管医院;
【基金】：国家自然科学基金面上项目(81571689)
【分类号】：R445.1

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