Carba NP试验检测产碳青霉烯酶肠杆菌科细菌和鲍氏不动杆菌的应用价值研究

发布时间：2018-02-21 09:28

本文关键词： Carba NP试验碳青霉烯酶碳青霉烯酶基因　出处：《中华医院感染学杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的评价Carba NP试验用于产碳青霉烯酶肠杆菌科细菌和鲍氏不动杆菌的应用价值。方法将2014年-2015年临床分离的耐碳青霉烯类抗菌素肠杆菌科细菌39株和鲍氏不动杆菌37株,采用Carba NP试验检测碳青霉烯酶;并采用PCR方法检测碳青霉烯酶相关基因,评价该试验的灵敏度和特异性。结果 26株肠杆菌科细菌PCR检出碳青霉烯酶相关基因,Carba NP试验检测肠杆菌科的敏感性、特异性、阳性预测值与阴性预测值分别为96.1%、94.8%、92.5%和97.3%;21株鲍氏不动杆菌PCR检出碳青霉烯酶相关基因,Carba NP试验检测鲍氏不动杆菌的敏感性、特异性、阳性预测值与阴性预测值分别为4.7%、100.0%、100.0%和45.9%。结论Carba NP试验能快速检出携带KPC、NDM-1、IMP-1和VIM-2基因肠杆菌科细菌的碳青霉烯酶,未能检出携带OXA-23基因的鲍氏不动杆菌的碳青霉烯酶。
[Abstract]:Objective to evaluate the application value of Carba NP test for carbapenem producing Enterobacteriaceae and Acinetobacter baumannii. Methods 39 strains of Enterobacteriaceae and 37 strains of Acinetobacter baumannii were isolated from 2014 to 2015. Carba NP test was used to detect carbapenase, and PCR method was used to detect carbapenase related genes. The sensitivity and specificity of the test were evaluated. Results the sensitivity and specificity of PCR detection of carbapenase related gene in 26 strains of Enterobacteriaceae were detected by Carba-NP assay. The positive predictive value and negative predictive value were 92.5% and 97.3%, respectively. The sensitivity and specificity of the Carba-NP test for the detection of carbapenase related genes in 21 strains of Acinetobacter baumannii were detected by Carba NP test. The positive predictive value and the negative predictive value were 4.70% and 45.9%, respectively. Conclusion Carba NP test can quickly detect carbapenase of Acinetobacter baumannii carrying KPC-NDM-1IMP-1 and VIM-2 genes, but not of Acinetobacter baumannii carrying OXA-23 gene.
【作者单位】：广州医科大学附属广州市第一人民医院检验科;
【基金】：国家自然科学基金资助项目(81201163) 广东省医学科研基金资助项目(B2014341) 广东省自然科学基金资助项目(2014A030313677)
【分类号】：R446.5

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