磁共振GRE电影序列评价恒河猴心功能的重复性及有效性研究

发布时间：2018-02-23 11:46

本文关键词： 心脏功能试验恒河猴磁共振成像　出处：《中国临床医学影像杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的 :对心脏磁共振梯度回波电影序列(Spoiled gradient echo,GRE)评估恒河猴心功能的有效性与重复性进行评价,为开展恒河猴疾病动物模型磁共振研究提供准确、最优的心功能评估策略。方法:正常恒河猴8只,均行超声心动图检查、心脏磁共振GRE电影序列扫描。采用心功能分析软件(cvi~(42))对GRE电影图像进行分析获取左室舒张末容积(EDV)、左室收缩末容积(ESV)、每搏量(SV)、射血分数(EF)、心输出量(CO)、舒张期左室质量(Myo Mass(Diast))以及收缩期左室质量(Myo Mass(Syst))等心功能相关参数值。进行磁共振扫描的复测实验,采用组内相关系数及Bland-Altman分析评价GRE各心功能参数的重复性,以超声心功能结果为相对的标准效度评价GRE各心功能参数的有效性,并对超声和GRE结果进行比较。结果:GRE重复性评价结果显示,各心功能参数的组内相关系数(ICC)值均在0.75~0.97之间,且显著性均小于0.01,重复性评价结果较好,Bland-Altman分析显示复测实验中各指标测量的一致性较高;有效性评价结果显示,除CO外,其余心功能参数的Pearson相关系数(r)值在0.80~0.92之间,具有统计学意义;同时发现GRE获取的EF、CO以及Myo Mass(Diast)要小于超声的测定值,差异具有统计学意义。结论:心脏磁共振GRE电影序列评价正常恒河猴心功能的结果具有较好的有效性与重复性,能有效、稳定地对恒河猴的心功能予以评价。
[Abstract]:Objective: to evaluate the validity and reproducibility of cardiac magnetic resonance gradient echo sequence (GREE) in evaluating the cardiac function of rhesus monkeys, so as to provide an accurate magnetic resonance study for animal model of rhesus monkey disease. Methods: 8 normal rhesus monkeys were examined by echocardiography. Cardiac magnetic resonance (GRE) film sequence scanning. Using cardiac function analysis software to analyze the GRE image to obtain left ventricular end-diastolic volume (EDV), left ventricular end-systolic volume (LVV), left ventricular end-systolic volume (LVEV), ejection fraction (EF), cardiac output volume (GRE), left ventricular diastolic volume (LV), left ventricular volume (LVEF), ejection fraction (EF), cardiac output volume (GRE) and diastolic left ventricle (LVV). Cardiac function parameters, such as Myo Massa Diasta and left ventricular mass in systolic phase, were measured by magnetic resonance imaging (MRI). The repeatability of cardiac function parameters in GRE was evaluated by intra-group correlation coefficient and Bland-Altman analysis. The validity of cardiac function parameters of GRE was evaluated by the relative standard validity of echocardiographic cardiac function. Results the repeatability of GRE was compared with that of ultrasound. Results the results of repetitive evaluation showed that the intra-group correlation coefficient of each cardiac function parameter was 0.75 ~ 0.97, respectively. The results of repeated Bland-Altman analysis showed that there was a higher consistency of the measurements in the repeated test, and the validity evaluation showed that the Pearson correlation coefficient (r) of the other cardiac function parameters was between 0.80 and 0.92, except for CO. At the same time, it was found that the GRE obtained EFCO and Myo Massi Diasta were smaller than the ultrasonic measurements. Conclusion: the results of cardiac magnetic resonance imaging (GRE) in evaluating the cardiac function of normal rhesus monkeys are effective and reproducible, and can be used to evaluate the cardiac function of rhesus monkeys stably.
【作者单位】：四川大学华西医院放射科;四川普莱美生物科技集团有限公司;圣路易斯华盛顿大学放射科;
【基金】：国家自然科学基金(81520108014,81528009) 重大科技计划973计划(2014CB541600) 四川省科技厅国际合作项目(2017HH0045)
【分类号】：R445.2;R540.4

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