表面增强拉曼光谱快速检测尿液中的尿酸

发布时间：2018-02-27 16:31

本文关键词： 表面增强拉曼光谱尿酸金纳米粒子　出处：《光谱学与光谱分析》2017年06期 　论文类型：期刊论文

【摘要】：表面增强拉曼光谱是一种表面灵敏度极高的"指纹"光谱技术,检测限可达单分子级别。它可以实现痕量物质的特异性识别及快速、无损检测,广泛应用于生命科学、电化学、环境安全等领域以及人们的日常生活中。通过种子生长法成功地实现了形貌均匀、尺寸可调的球形金纳米粒子的制备,并以此作为增强基底进一步探索其粒径对尿酸拉曼谱峰强度的影响。结果表明,金纳米粒子的尺寸显著影响其拉曼增强能力。在研究范围内,随着金纳米粒子尺寸的增加,其拉曼增强能力逐渐增加。在激光波长为638nm时,150nm的金纳米粒子具有最优的拉曼增强能力。这使得它们可适用于尿酸溶液的快速高灵敏度分析,检测限可达0.01mmol·L~(-1)。进一步的研究还表明,该方法可用于痕量尿酸的定量检测。在0.01~0.5mmol·L~(-1)范围内,尿酸的浓度与其特征拉曼峰640cm~(-1)处的峰强度之间呈线性关系,线性相关系数达0.98。将该方法用于真实样品(正常人体尿液)的快速检测,发现该方法不受尿液中其他成分的干扰,可以实现人体尿液中尿酸含量的快速测定。研究结果表明,以金纳米粒子作为基底的表面增强拉曼光谱方法可方便、快速地对尿液中尿酸的含量进行分析,极大地拓展了表面增强拉曼光谱在临床上的应用与研究。
[Abstract]:Surface-enhanced Raman spectroscopy is a highly sensitive "fingerprint" spectroscopic technique with a detection limit of up to a single molecular level. It can realize the specific recognition and rapid nondestructive detection of trace substances, and is widely used in life sciences and electrochemistry. In the field of environmental safety and people's daily life, the spherical gold nanoparticles with uniform morphology and adjustable size were successfully prepared by seed growth method. The results showed that the size of gold nanoparticles significantly affected their Raman enhancement ability. In the range of study, the size of gold nanoparticles increased with the increase of the size of gold nanoparticles. The gold nanoparticles with laser wavelength of 638 nm have the optimum Raman enhancement ability, which makes them suitable for rapid and high sensitivity analysis of uric acid solution. The detection limit can be up to 0.01 mmol 路L ~ (-1). Further studies show that the method can be used for quantitative determination of trace uric acid. In the range of 0.01U _ (0.5 mmol 路L ~ (-1)), there is a linear relationship between the concentration of uric acid and the peak intensity at the characteristic Raman peak of 640 cm ~ (-1). The linear correlation coefficient is 0.98. The method has been applied to the rapid detection of real samples (normal human urine). It is found that the method can be used to determine the uric acid content in human urine without interference from other components in the urine. The method of surface-enhanced Raman spectroscopy based on gold nanoparticles is convenient and rapid to analyze uric acid in urine, which greatly expands the clinical application and research of surface-enhanced Raman spectroscopy.
【作者单位】：浙江省人民医院耳鼻喉科杭州医学院附属人民医院;厦门大学固体表面物理化学国家重点实验室化学化工学院;
【基金】：浙江省医药卫生科技计划项目(2015KYB025) 国家自然科学基金项目(21522508)资助
【分类号】：O657.37;R446.12

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