医院环境中分枝杆菌污染检测方法研究

发布时间：2018-03-04 05:30

本文选题：医院环境　切入点：分枝杆菌　出处：《中国消毒学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的筛选医院呼吸科环境中分枝杆菌污染检测的适宜方法,为防控分枝杆菌医院感染提供参考依据。方法采用平板沉降法和微生物采样方法对江苏省某医院呼吸科环境空气和物体表面以及医疗器械进行采样监测与细菌分离鉴定。结果用平板沉降法和采样器以及气溶胶法共计采集空气样本52份,通过细菌分离培养法均未检出分枝杆菌。用棉拭子涂抹法采集气空调滤网、物体表面和支气管镜等样本共计16份,细菌分离培养也未检出分枝杆菌。气溶胶法采集的空气样本和空调滤网样本,采用线性探针基因杂交技术检测,分枝杆菌基因检出率达50.0%。结论采用常规微生物采样和细菌培养检测方法,在医院呼吸科空气、物体表面和支气管镜等样本中均未检出分枝杆菌,用线性探针基因杂交技术在空气和空调滤网上检出分枝杆菌基因。
[Abstract]:Objective to screen the suitable method for detection of mycobacterium contamination in respiratory department of hospital. Methods the methods of plate sedimentation and microbial sampling were used to sample and monitor the air, object surface and medical instruments of respiratory department in a hospital in Jiangsu province. Results A total of 52 air samples were collected by plate sedimentation, sampler and aerosol method. No mycobacterium was detected by bacterial isolation and culture. 16 samples were collected by cotton swab smear method, such as air conditioning screen, object surface and bronchoscope, etc. No mycobacterium was detected in bacteria isolation and culture. The air samples and air conditioning filter samples collected by aerosol method were detected by linear probe gene hybridization. Conclusion the detection rate of mycobacterium gene is 50.0%. Conclusion No mycobacteria was detected in the air, surface and bronchoscope of respiratory department of hospital by routine microbial sampling and bacterial culture detection. Mycobacterium genes were detected by linear probe gene hybridization in air and air conditioning filters.
【作者单位】：江苏省疾病预防控制中心;
【基金】：江苏省重大新发传染病综合防控科技示范工程(BE2015714) 江苏省卫生厅科技项目(Y2012086)
【分类号】：R446.5

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