肠炎沙门氏菌和哈瓦那沙门氏菌的耐药性及耐药基因分析

发布时间：2018-03-06 16:46

本文选题：肠炎沙门氏菌　切入点：哈瓦那沙门氏菌　出处：《现代食品科技》2017年10期 　论文类型：期刊论文

【摘要】：为了保障食品安全,更好的了解耐药性的产生及传播的途径,研究了新疆乌鲁木齐市部分农贸市场内检出的17株肠炎沙门氏菌和11株哈瓦那沙门氏菌的药敏性及相关耐药基因。用琼脂稀释法测定沙门氏菌的药敏性,用聚合酶链式反应和测定基因序列的方法确定耐药沙门氏菌中与喹诺酮类药物相关的抗性决定区突变基因以及质粒携带的耐药基因。17株肠炎沙门氏菌对环丙沙星和头孢西丁表现为100%敏感,对萘定酮酸的耐药率为94.1%,头孢曲松的耐药率为17.6%,qnr B检出率为64.7%,17株肠炎沙门氏菌发生Gyr A基因突变,主要突变类型为Asp87Tyr;11株哈瓦那沙门氏菌对甲氧倫啶、氯霉素、磺胺二甲异唑、磺胺甲基异恶唑/甲氧苄啶、萘啶酮酸、头孢西丁的耐药率为100%、63.6%、36.4%、18.2%、9.1%和9.1%,氨苄西林、阿莫西林/克拉维酸敏感率为100%,qnr B,qnr S的检出率均为9.1%,11株哈瓦那沙门氏菌Par C基因突变类型为Thr57ser。新疆乌鲁木齐肠炎沙门氏菌和哈瓦那沙门氏菌耐药情况比较严重,对抗生素的耐药状况应当予以关注,其喹诺酮类药物耐药决定区突变基因及质粒携带的耐药基因在一定程度上会影响肠炎沙门氏菌和哈瓦那沙门氏菌的耐药机制。
[Abstract]:In order to ensure food safety and better understand the generation and transmission of drug resistance, The drug sensitivity of 17 strains of Salmonella enteritidis and 11 strains of Havana Salmonella were studied in Urumqi, Xinjiang, China. The drug sensitivity of Salmonella enteritidis was determined by Agar dilution method. Identification of Quinolone-Related Resistance determining region Mutants in Drug resistant Salmonella by Polymerase chain reaction and Gene sequencing and plasmid carrying Resistance Gene .17 Salmonella enteritidis to ciprofloxacin. And cefoxitin showed sensitivity to 100%, The Gyr A gene mutation was found in 17 strains of Salmonella enteritidis. The main mutation types were: Asp87 Tyr-11 strains of salmonella Havana, methoxylamidine, chloramphenicol, sulfamethoxazole, and ceftriaxone, and the rate of resistance to ceftriaxone was 17.6%, and the positive rate was 64.77.The main mutation types were: Asp87 Tyr 11 strains of salmonella Havana, methoxylamidine, chloramphenicol, sulfamethoxazole. The resistance rate of sulfamethoxazole / trimethoprim, naphthalene ketoic acid and cefxitin was 100% and 63.6%. The sensitivity rate of amoxicillin / clavulanic acid was 100. The detectable rate of QNR was 9. 1%. The mutation type of Par C gene of 11 strains of Salmonella Havana was Thr57 ser.Salmonella enteritidis and Salmonella Havana were more resistant in Urumqi, Xinjiang. The resistance of antibiotics should be paid attention to. The mutant genes of quinolone drug resistance region and the drug resistance genes carried by plasmids may influence the resistance mechanism of Salmonella enteritidis and Salmonella Havana to some extent.
【作者单位】：新疆农业大学食品科学与药学学院;新疆维吾尔自治区科技项目服务中心;
【基金】：新疆维吾尔自治区“十三五”重大专项项目“新疆民族特色食品品质分析及技术标准研究”(2016A01001-5)
【分类号】：R446.5

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