消化后AB-PAS染色法在黏液染色中的应用

发布时间：2018-03-18 18:36

本文选题：消化后AB-PAS　切入点：黏液染色　出处：《临床与实验病理学杂志》2017年07期 　论文类型：期刊论文

【摘要】：正AB-PAS是一种常见的特殊染色法,其能较好地显示中性黏液和酸性黏液,但有时组织内含有的糖原也会显示,且无法区分中性黏液,从而影响染色结果的判断。本科室根据糖原可被淀粉酶分解破坏的原理~([1]),在染色前用淀粉酶消化切片中的糖原,再进行A B-PAS染色,取得良好的效果,现介绍如下。1材料与方法1.1标本来源收集深圳市人民医院病理科已知AB-PAS染色阳性标本,每份标本石蜡切片2张,厚4μm,分为两组:(1)常规AB-PAS染色(对照组),(2)消化后行AB-PAS染色(实验组)。
[Abstract]:Positive AB-PAS is a special staining method, which can display neutral mucus and acidic mucus well, but sometimes the glycogen in tissues also shows, and can not distinguish neutral mucus. According to the principle that glycogen can be destroyed by amylase, the glycogen was digested by amylase before dyeing, and then stained with AB-PAS. This paper introduces the following 1. 1 materials and methods 1. 1 specimens collected from the Department of Pathology of Shenzhen people's Hospital, 2 paraffin sections per specimen, known to be positive for AB-PAS staining. Four 渭 m thick, divided into two groups: 1) routine AB-PAS staining (control group 2) and AB-PAS staining after digestion (experimental group).
【作者单位】：深圳市人民医院病理科;广州军区广州总医院病理科;
【分类号】：R361.2

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