2014-2015年银川市24株风疹病毒基因型流行特征分析

发布时间：2018-03-24 10:01

本文选题：风疹病毒　切入点：基因型　出处：《现代预防医学》2017年16期

【摘要】：目的对2014-2015年银川市流行的风疹病毒进行分离鉴定,确定其基因型,并进行基因与氨基酸的特征分析。方法采用Real-time PCR法对采集的疑似风疹病例的咽拭子标本进行初筛,阳性标本使用Vero/SLAM细胞进行病毒分离,采用RT-PCR法对病毒培养物进行风疹病毒株E1基因的标准靶核苷酸序列扩增,并对扩增产物进行序列测定与分析。结果 2014-2015年银川市共分离到24株风疹病毒株,进化树结果显示均为2B基因型;24株分离株之间的核苷酸与氨基酸同源性分别为98.1%~100.0%和97.5%~100.0%。对核苷酸编码的氨基酸序列进行比对,尽管出现了多个氨基酸的突变位点,但关键位点如N-型糖基化位点、血凝抑制位点、中和位点以及重要的抗原表位均未发生变化。结论 2014-2015年银川市流行的风疹病毒主要是2B基因型,与国内其他地区普遍流行的基因型(1E基因型)不一致。虽然出现了氨基酸的变异,但关键位点的氨基酸序列高度保守,病毒抗原性较稳定。
[Abstract]:Objective to isolate and identify the rubella virus prevalent in Yinchuan from 2014 to 2015, to determine its genotype and to analyze the characteristics of gene and amino acid. Methods the throat swab samples of suspected rubella cases were screened by Real-time PCR method. The positive specimens were isolated by Vero/SLAM cells, and the standard target nucleotides of rubella virus E1 gene were amplified by RT-PCR method. The amplified products were sequenced and analyzed. Results 24 strains of rubella virus were isolated from Yinchuan City in 2014-2015. The results of phylogenetic tree showed that the nucleotide and amino acid homology were 98.1% and 97.5%, respectively. The amino acid sequences encoded by nucleotides were compared, although there were several amino acid mutation sites. But the key sites, such as N- type glycosylation sites, hemagglutination inhibition sites, neutralizing sites and important antigen epitopes, have not changed. Conclusion the rubella virus prevalent in Yinchuan City in 2014-2015 is mainly a 2B genotype. Although there were amino acid variations, the amino acid sequences of the key sites were highly conserved and the antigenicity of the virus was relatively stable.
【作者单位】：银川市疾病预防控制中心;银川市卫生与计划生育委员会;宁夏疾病预防控制中心;
【分类号】：R440;R511.2

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