携尿激酶靶向微泡在超声介导下对兔股动脉溶栓的作用

发布时间：2018-03-24 14:35

本文选题：血栓栓塞　切入点：股动脉　出处：《中国医学影像学杂志》2017年08期

【摘要】：目的观察不同超声频率联合携尿激酶的靶向微泡造影剂对兔股动脉血栓的溶解作用,探讨影响溶栓作用的主要因素,寻找微循环再栓塞的相关指标。材料与方法 72只新西兰大白兔制作单侧股动脉血栓模型,按照完全随机分组方法分为12组,每组6只。根据超声频率(1.6、2.2、2.8 MHz)、超声照射时间(30、60 min)、尿激酶剂量(3、6 mg)3因素不同水平进行实验组合。靶向微泡携带尿激酶在低频超声辅助照射下溶栓,观察血管的溶通情况,并通过HE染色证实有无微循环再栓塞。抽取兔血检测6-酮-前列腺素Fla(6-keto-PGF1a)、血栓素B2(TXB2)、P/T比值(6-keto-PGF1a/TXB2)及P-选择素(SP)等指标。结果超声频率2.2 MHz、超声照射时间30 min、尿激酶剂量3 mg组血管全部溶通且无微栓塞发生;其余各组均有未溶通或合并微循环再栓塞发生的情况。溶栓后无微栓塞组的兔6-keto-PGF1a含量明显升高,差异有统计学意义(P0.05);而其他指标差异无统计学意义(P0.05)。结论超声频率2.2 MHz、超声照射时间30 min、尿激酶低剂量3 mg的条件下溶栓可实现血管的完全溶通。超声频率、超声照射时间及尿激酶剂量一定时可有效溶解血栓,但在溶栓的过程中可能会发生微循环的再栓塞。6-keto-PGF1a含量的升高对降低微循环再栓塞具有一定作用。
[Abstract]:Objective to observe the effect of different ultrasound frequency combined with urokinase targeted microbubble contrast agent on thrombolysis of rabbit femoral artery, and to explore the main factors affecting thrombolytic effect. Materials and methods A unilateral femoral artery thrombosis model was made in 72 New Zealand rabbits. There were 6 rats in each group. According to the ultrasonic frequency of 1.6m 2.2U 2.8 MHz, the time of ultrasound irradiation was 300.60 min, and the dose of urokinase was 36 ~ 6 mg)3, the thrombolytic effect of urokinase was observed by low-frequency ultrasound irradiation, and the thrombolytic effect was observed. The results showed that there was no microcirculation reembolization by HE staining. The blood samples were extracted to detect 6-keto-PGF1aI, 6-keto-PGF1a / TXB2 / TXB2.Results Ultrasound frequency was 2.2 MHz, ultrasound irradiation time was 30 min, urokinase dose was 3 min, and 6-keto-PGF1a / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2 / TXB2, respectively. In mg group, all the vessels were dissolved and no microembolization occurred. After thrombolytic therapy, the content of 6-keto-PGF1a in the non-thrombolytic group was significantly increased. Conclusion Ultrasound frequency 2.2 MHz, ultrasound irradiation time 30 min, urokinase low dose 3 mg thrombolytic thrombolysis can realize the complete thrombolysis of blood vessel, and the ultrasonic frequency can be achieved under the condition of low dose of urokinase, while there is no significant difference in other indexes (P 0.05 and P 0.05), conclusion the ultrasound frequency is 2.2 MHz, the time of ultrasound irradiation is 30 min, and the dosage of urokinase is 3 mg. Ultrasound irradiation time and urokinase dose can effectively dissolve thrombus, but the increase of microcirculation reembolization may occur during thrombolysis. The increase of 6-keto-PGF1a content may play a certain role in decreasing microcirculation reembolization.
【作者单位】：新疆医科大学第一附属医院心脏超声诊断科;
【基金】：国家自然科学基金(81301230)
【分类号】：R445.1;R541.4

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