骨髓间充质干细胞移植在卵巢早衰小鼠卵巢功能重建中应用

发布时间：2018-04-01 16:11

本文选题：骨髓间充质干细胞　切入点：干细胞移植　出处：《安徽农业大学学报》2017年01期

【摘要】：探讨骨髓间充质干细胞(BMSCs)移植在卵巢早衰小鼠卵巢功能重建中的应用价值。通过一次性腹腔注射环磷酰胺和白消安建立小鼠卵巢早衰(POF)模型,并随机分为建模组、BMSCs移植组和空白对照组。移植组于建模14 d经尾静脉注射绿色荧光蛋白(GFP)转基因鼠BMSCs悬液(5×10~7个·mL~(-1)),每4 d重复1次,共5次;建模组和空白对照组注射等量生理盐水。观察建模后及BMSCs移植后小鼠的一般情况、血清FSH和E2水平、卵巢组织学变化以及GFP荧光信号在卵巢内的分布。结果发现,建模组小鼠食欲明显减退,活动减少,与空白对照组相比体重显著减轻(P0.05),FSH水平显著增高(P0.05),E2水平显著降低(P0.05),卵巢体积减小,间质纤维化严重,始基卵泡、生长卵泡和成熟卵泡数均显著减少(P0.05)。BMSCs移植后,移植组小鼠体重增加,食欲增强,活动增多;28 d后FSH浓度显著低于建模组(P0.05),E2浓度、始基卵泡、生长卵泡数及成熟卵泡数均显著高于建模组(P0.05),但各项指标仍与空白对照组间存在显著性差异;荧光显微镜下可见绿色荧光信号散在分布于卵泡周围。此结果提示,BMSCs可在小鼠损伤卵巢组织中定位存活,对卵巢组织结构及内分泌功能有修复作用。
[Abstract]:To investigate the application value of bone marrow mesenchymal stem cells (BMSCs) transplantation in ovarian function reconstruction of premature ovarian failure mice, the model of premature ovarian failure (POF) was established by intraperitoneal injection of cyclophosphamide (cyclophosphamide) and BMSCs. The BMSCs suspension of GFP transgenic mice was injected into caudal vein for 14 days, and it was repeated once every 4 days for 5 times. The normal saline was injected into the model group and the blank control group. The general situation, the serum FSH and E2 levels, the histological changes of ovary and the distribution of fluorescence signal of GFP in the ovary were observed after modeling and after BMSCs transplantation. In the model group, the appetite decreased and the activity decreased. Compared with the blank control group, the body weight of the model group decreased significantly. Compared with the control group, the level of P0.05FSH increased significantly. The level of E2 decreased significantly. The volume of ovaries decreased, the interstitial fibrosis was serious, and the primary follicles were also decreased. The number of growth follicles and mature follicles decreased significantly after transplantation, the body weight and appetite were increased in the transplanted group, and the FSH concentration was significantly lower than that in the model group at 28 days after transplantation, and the primary follicles were significantly lower than those in the model group. The number of growth follicles and mature follicles were significantly higher than that of the model group (P0.05A), but there were significant differences between the indexes of the model group and the blank control group. The green fluorescence signal was scattered around the follicles under the fluorescence microscope. The results suggested that BMSCs could survive in the damaged ovary tissue and repair the structure and endocrine function of the ovary.
【作者单位】：解放军第105医院生殖医学中心;
【基金】：南京军区医学科技创新课题(11Z010)资助
【分类号】：R711.75;R457.7

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