帕瑞昔布钠对脓毒症小鼠肠黏膜屏障的保护作用

发布时间：2018-04-03 04:00

本文选题：帕瑞昔布钠　切入点：脓毒症　出处：《临床麻醉学杂志》2017年11期

【摘要】：目的研究帕瑞昔布钠对脓毒症小鼠肠黏膜屏障功能的影响及可能机制。方法 21只C57BL/6小鼠随机分为三组:假手术组(Sham组)、脓毒症模型组(CLP组)、脓毒症模型+帕瑞昔布钠2mg/kg治疗组(P组),每组7只。术后24h用襻环结扎法检测各组小鼠小肠通透性。另21只C57BL/6小鼠随机分为三组,分组及治疗同前,术后24h处死小鼠,收集小鼠回肠。小肠组织行HE染色观察肠病理损伤。采用Western bolt法检测小肠ZO-1、Occludin、Claudin-1等紧密连接蛋白的表达。采用ELISA法检测小肠黏膜IL-6、PGE2的浓度。结果与Sham组比较,CLP组小鼠小肠明显损伤,门静脉血内FD4浓度明显升高,小肠黏膜内ZO-1、Occludin、Claudin-1蛋白表达明显减少、IL-6与PGE2浓度明显升高(P0.05)。与CLP组比较,P组小鼠小肠损伤明显减轻(P0.05),门静脉血内FD4浓度明显降低(P0.05),小肠黏膜内各蛋白表达水平明显增加、IL-6与PGE2浓度明显降低(P0.05)。结论帕瑞昔布钠治疗可以明显降低脓毒症导致的肠黏膜屏障功能损伤,其机制可能与降低肠组织炎症水平,增加肠紧密连接蛋白表达相关。
[Abstract]:Objective to study the effect of paroxib sodium on intestinal mucosal barrier function in septic mice and its possible mechanism.Methods Twenty-one C57BL/6 mice were randomly divided into three groups: sham group (sham group), sepsis model group (C57BL/6 group), paruoxib sodium 2mg/kg group (n = 7).The permeability of small intestine in each group was detected by loop ligation 24 hours after operation.The other 21 C57BL/6 mice were randomly divided into three groups. The mice were killed 24 hours after operation and the ileum was collected.The intestinal pathological injury was observed by HE staining.Western bolt method was used to detect the expression of cladin-1 and other tight junction proteins in the small intestine.The concentration of IL-6 and PGE2 in small intestinal mucosa was detected by ELISA method.Results compared with the Sham group, the small intestine injury and the FD4 concentration in the portal vein were significantly increased, and the expression of Claudin-1 protein in the intestinal mucosa was significantly decreased. The levels of IL-6 and PGE2 were significantly increased (P 0.05).Compared with CLP group, the injury of small intestine in P group was significantly alleviated, the concentration of FD4 in portal vein blood was significantly decreased, and the expression level of various proteins in intestinal mucosa was significantly increased. The levels of IL-6 and PGE2 in small intestine were significantly decreased (P 0.05).Conclusion Pareoxib sodium therapy can significantly reduce the intestinal mucosal barrier function damage induced by sepsis, and its mechanism may be related to the reduction of intestinal inflammation and the increase of intestinal tight junction protein expression.
【作者单位】：广州军区广州总医院麻醉科;
【基金】：国家自然科学基金(81501647) 广东省科技计划项目(2014A020212555)
【分类号】：R459.7

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