输注聚合人胎盘血红蛋白对大鼠氧化损伤的初步研究

发布时间：2018-04-12 08:45

  本文选题：氧化损伤 + 聚合人胎盘血红蛋白　； 参考：《中国输血杂志》2017年04期

【摘要】：目的探讨输注不同浓度聚合人胎盘血红蛋白(Poly PHb)对大鼠造成的氧化损伤。方法建立动物换血模型,将50只SD大鼠随机均分为假手术组:仅做股动/静脉插管处理;氯化钠注射液(对照)组:股动/静脉插管,输注氯化钠注射液行换血实验;2%、4%及6%Poly PHb 3个实验组:股动/静脉插管,分别输注相应浓度的Poly PHb行换血实验。分别在大鼠换血前、换血70%后即刻观测平均动脉压(MAP),换血完成后即刻从大鼠股动脉取血0.5 m L/只,做动脉血血气分析,并检测各组大鼠血浆中还原型谷胱甘肽(GSH)浓度、超氧化物歧化酶(SOD)活力、总抗氧化能力(T-AOC)、丙二醛(MDA)浓度、8-羟基脱氧鸟苷(8-OHDG)浓度。结果 1)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,GSH(μmol/L)分别为33.76±14.33、47.98±21.88、39.73±15.54及10.25±5.70(P0.01);SOD(U/m L)分别为238.07±27.39、355.73±35.42、405.26±45.01及138.46±57.16(P0.01);T-AOC(U/m L)分别为20.50±7.87、32.65±4.38、38.03±4.50及5.40±2.22(P0.01)。2)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,MDA浓度(nmol/m L)随着Poly PHb浓度的增高而增高:45.08±4.12、54.39±3.16、58.52±6.33及2.85±0.59(P0.01)。3)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,大鼠血浆8-OHDG浓度(nmol/m L)未发生明显变化:2.54!0.90、2.76!0.99、2.79!0.82及2.26!0.42(P0.05)。结论 Poly PHb对大鼠造成脂质过氧化损伤,且氧化损伤程度随着Poly PHb浓度的增高而加重,但并未对大鼠DNA造成损伤。
[Abstract]:Objective to investigate the oxidative damage induced by different concentrations of polymerized human placental hemoglobin (Poly PHb) in rats.Methods 50 SD rats were randomly divided into sham-operation group (femoral artery / vein intubation only) and sodium chloride injection (control) group (femoral / venous intubation).Three experimental groups: femoral artery / vein catheterization, transfusion of sodium chloride injection with 4% and 6%Poly PHb, and transfusion of corresponding concentration of Poly PHb were performed.The mean arterial pressure (MAPH) was measured immediately before and 70% after the exchange of blood in rats, and 0.5 mL of blood was taken from the femoral artery of rats immediately after the exchange of blood. The arterial blood gas was analyzed and the plasma GSH concentration of reduced glutathione was measured in each group.Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), malondialdehyde (MDA) concentration, 8-hydroxydeoxyguanosine (8-OHDG) concentration.缁撴灉 1)澶ч紶鎹㈣��70%鍚，

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