脐带间充质干细胞对NB4白血病细胞基因组表达谱的影响

发布时间：2018-04-13 05:10

本文选题：脐带 + 间质干细胞　；参考：《中国组织工程研究》2017年25期

【摘要】：背景:间充质干细胞是体内微环境的重要组成部分且影响肿瘤细胞的多种生物学行为,间充质干细胞的潜在临床价值是近年来的研究热点。目的:通过基因芯片方法分析脐带间充质干细胞对急性早幼粒细胞白血病细胞系NB4细胞基因组表达谱的影响。方法:体外建立脐带间充质干细胞与急性早幼粒细胞白血病细胞系NB4细胞共培养体系,检测脐带间充质干细胞对NB4细胞增殖、凋亡和分化的影响,分别提取了NB4细胞单独培养组和NB4细胞+脐带间充质干细胞共培养组NB4细胞的mRNA并将其反转录为cDNA,两组分别标记荧光,做成探针并与基因芯片杂交,检测其荧光强度,分析两组基因表达谱的差异。结果与结论:(1)脐带间充质干细胞促进NB4细胞的增殖和分化并抑制其凋亡;(2)脐带间充质干细胞作用后的NB4细胞的基因表达谱中有530个基因上调和53个基因下调,与基因相关的功能和信号通路也在一定程度上受到了调节;(3)结果表明,脐带间充质干细胞通过改变肿瘤细胞内大量基因表达、基因功能及多种信号通路影响NB4细胞的生物学行为。
[Abstract]:Background: mesenchymal stem cells are an important part in the micro environment and the influence of various biological behavior of tumor cells, mesenchymal stem cells and its potential clinical value is a hot research topic in recent years. Objective: to analyze the umbilical cord mesenchymal stem cells on acute promyelocytic leukemia cells NB4 gene expression profiles affected by the gene chip method. Methods: human umbilical cord mesenchymal stem cells and acute promyelocytic leukemia cell line NB4 cells co culture system in vitro, detection of umbilical cord mesenchymal stem cells on NB4 cell proliferation, apoptosis and differentiation, were extracted and NB4 + cells in human umbilical cord mesenchymal stem cells co cultured NB4 cells mRNA and reverse transcribed into cDNA NB4 cells were cultured alone, two groups were labeled with a fluorescent probe, and made hybridization and gene chip, detecting the fluorescence intensity, analysis of two groups of gene expression difference ISO. Results and conclusion: (1) human umbilical cord mesenchymal stem cells promote the proliferation and differentiation of NB4 cells and inhibit their apoptosis; (2) human umbilical cord mesenchymal stem cells after NB4 cell gene expression profiles of 530 genes up-regulated and 53 genes were down regulated, and the signal pathway and gene function related to a certain extent by the regulation; (3) the results showed that the umbilical cord mesenchymal stem cells by altering the expression of a large number of genes in tumor cells, gene function and signaling pathways of biological behavior of NB4 cells.

【作者单位】：中国医学科学院北京协和医学院血液学研究所血液病医院实验血液学国家重点实验室;
【基金】：国家自然科学基金(81500098和81330015) 中国医学科学院医学与健康科技创新工程(2016-I2M-1-017)~~
【分类号】：R457.7;R733.7

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