近红外散射光谱的组织热毁损程度实时鉴别

发布时间：2018-04-16 17:15

  本文选题：激光热毁损 + 离体猪肝　； 参考：《光谱学与光谱分析》2017年10期

【摘要】：提出了利用近红外散射光谱对生物组织热毁损程度进行实时鉴别的一种新方法。实验首先分别用功率3.5,5,6.5和8 W的808nm脉冲激光对新鲜离体猪肝进行热毁损,在距离毁损中心10mm处实时采集近红外散射光谱和温度,以最小二乘法拟合得到波长830~900nm处的光谱斜率(S830~900),将其不同上升幅度为条件结束加热。其次,对光谱采集点附近的组织作病理切片,根据组织学特征进行评分,采用3级评分制(3分为完全毁损,2分为部分毁损,1分为无效毁损)评估毁损程度。最后,分析加热期S830~900最大上升幅度、降温后S830~900最终稳定值与病理分析的毁损程度之间的关联。结果表明,一般情况下,若加热期S830~900上升至初值的4倍以上、降温后最终稳定于3.5倍以上,对应组织为完全毁损;若S830~900加热期上升2~5倍,降温后最终稳定于1~1.5倍,对应组织为无效毁损;其余为部分毁损。根据分析结果,首次建立了光谱因子与热毁损程度的关联,利用加热期S830~900的最大上升幅度与降温后的最终稳定值来鉴别组织毁损程度,可以很好的降低实验对象个体差异性对判断结果的影响,具有很好的鉴别效果,其准确率达到90%以上,实现了组织热毁损程度实时、准确的鉴别,为组织热毁损实时监控提供了一种新思路。
[Abstract]:A new method for real time identification of thermal damage of biological tissues by near infrared scattering spectroscopy is proposed.In this experiment, the fresh isolated porcine liver was damaged by 808nm pulse laser with power of 3.5 and 8 W, respectively. The near infrared scattering spectra and temperature were collected in real time at the 10mm distance from the damage center.The spectral slope of the wavelength 830~900nm was obtained by least square fitting, and the different rising amplitude was used as the condition to end the heating.Secondly, histopathological sections were made in the vicinity of the spectral sampling points. According to the histological characteristics, the degree of damage was evaluated by using a 3-grade scoring system, which was divided into complete damage and partial damage, divided into partial damage and partial damage, and divided into invalid damage.Finally, the relationship between the final stable value of S830T900 during heating period and the damage degree of pathological analysis was analyzed.The results show that, under normal conditions, if S830T900 rises to more than 4 times of initial value during heating period, it will eventually stabilize at 3.5 times after cooling, and the corresponding tissue will be destroyed completely, and if S830T900 increases 25 times during heating period, after cooling, it will eventually stabilize at 11. 5 times.The corresponding organization is invalid damage; the rest is partial damage.Based on the results of the analysis, the correlation between the spectral factor and the degree of thermal damage was established for the first time. The maximum rise of S830T900 during the heating period and the final stable value after cooling were used to identify the degree of tissue damage.It can reduce the influence of the individual difference on the result of the experiment, and has a good discriminant effect. The accuracy rate is over 90%, which realizes the real-time and accurate identification of the degree of tissue thermal damage.It provides a new idea for real-time monitoring of tissue thermal damage.
【作者单位】： 南通大学机械工程学院;南京航空航天大学生物医学工程系;
【基金】：国家自然科学基金项目(61405098)资助
【分类号】：O657.3;R45
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本文编号：1759859