整合素αvβ3靶向双模态分子探针USPIO-cy5.5-cRGD的制备与表征

发布时间：2018-04-19 06:16

本文选题：双模态探针 + 磁共振成像　；参考：《临床放射学杂志》2017年03期

【摘要】：目的合成整合素αvβ3靶向的磁共振(MRI)/近红外荧光成像(NIRF)双模态分子探针,并初步研究其粒径、稳定性、荧光偶联情况及MRI表现。方法高温热解法合成Fe_3O_4纳米颗粒(超小超顺磁性氧化铁,US-PIO),先在其表面包覆聚乙二醇(PEG)和近红外荧光染料cy5.5,再进一步偶联整合素αvβ3靶向配体环状RGD多肽(cRGD)后得到双模态分子探针USPIO-cy5.5-cRGD。之后用透射电镜检测其粒径,动态光散射法检测其水动力尺寸和zeta电位,紫外可见分光光度法检测其荧光偶联情况。运用全能分子分析系统观察USPIO-cy5.5-cRGD荧光成像表现、3.0 T MRI观察其MR成像表现。结果USPIO-cy5.5-cRGD制备成功,测得Fe_3O_4纳米颗粒直径为(10.08±0.34)nm;USPIO-cy5.5-cRGD的水动力尺寸为(46.66±16.31)nm,zeta电位为(-53.87±1.99)mV,在波长685 nm处有最大吸收峰;随着USPIO-cy5.5-cRGD的浓度逐步升高,荧光强度增加,T_2信号强度逐渐降低,经线性拟合得到横向弛豫率r_2为251.55。结论双模态分子探针USPIO-cy5.5-cRGD具有良好的分散性、稳定性,可进行荧光显像及显著降低T_2信号。
[Abstract]:Objective to synthesize integrin 伪 v 尾 3 targeted bimodal molecular probes, and to study their particle size, stability, fluorescence coupling and MRI performance.Methods Fe_3O_4 nanoparticles (ultrasmall ultraparamagnetic ferric oxide US-PIOD) were synthesized by high temperature pyrolysis. PEG and cy5.5 were first coated on the surface, and then the integrin 伪 v 尾 3 targeted ligand cyclic RGD polypeptide cRGDwas further coupled to obtain a bimodular molecular probe USPIO-cy5.5-cRGD.The particle size was measured by transmission electron microscope (TEM), the hydrodynamic size and zeta potential were measured by dynamic light scattering (DLS), and the fluorescence coupling was detected by UV-Vis spectrophotometry.The USPIO-cy5.5-cRGD fluorescence imaging manifestations were observed by using the omnipotent molecular analysis system. The 3. 0 T MRI was used to observe the Mr imaging manifestations.Results USPIO-cy5.5-cRGD was successfully prepared. The diameter of Fe_3O_4 nanoparticles was 10.08 卤0.34nmg-cy5.5-cRGD. The hydrodynamic size of USPIO-cy5.5-cRGD was 46.66 卤16.31nmmV, with the maximum absorption peak at 685nm, and the intensity of T2 signal decreased with the increase of USPIO-cy5.5-cRGD concentration.By linear fitting, the transverse relaxation rate r _ s _ 2 is 251.55.Conclusion bimodular molecular probe USPIO-cy5.5-cRGD has good dispersion and stability. It can be used for fluorescence imaging and can significantly reduce the signal of T _ 2.
【作者单位】：广西医科大学研究生学院;广西医科大学附属肿瘤医院影像中心;
【基金】：国家自然科学基金资助项目(编号:81460452)
【分类号】：R445.2;R73

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