海马区血小板活化因子合酶及其受体的表达对选择性神经损伤大鼠痛觉敏化的影响

发布时间：2018-04-24 06:16

本文选题：银杏内酯B + 神经病理性疼痛　；参考：《华中科技大学学报(医学版)》2017年04期

【摘要】：目的探讨海马区血小板活化因子(PAF)合酶(LPCAT1、LPCAT2)及其受体(PAFR)在大鼠坐骨神经分支选择性神经损伤(SNI)所致神经病理性疼痛中的作用。方法选择海马区置管成功的雄性SD大鼠40只,随机分为4组(n=10):假手术组(Sham组),模型组(SNI组),模型+银杏内酯B组(SNI+BN52021组)和溶剂对照组(SNI+HBC组)。SNI+BN52021组在SNI手术后2d通过海马区微量注射给予银杏内酯B 10μg(溶解于1μL的HBC溶液),SNI+HBC组则给予1μL的HBC溶液。分别于术前1d和术后1、3、5、7、10、14d记录各组动物的机械缩爪阈值(PWMT)和辐射热缩爪潜伏期(PWTL);第15天取大鼠新鲜海马组织,RT-PCR检测LPCAT1、LPCAT2以及PAFR的表达。结果SNI大鼠PWMT值术后第1天开始明显降低(P0.05),海马组织中LPCAT2及PAFR的表达增强(均P0.05),LPCAT1的表达变化不明显(P0.05);海马区微量注射BN52021组大鼠的PWMT值升高,海马组织中LPCAT2及PAFR的表达下降(均P0.05)。结论海马区微量注射银杏内酯B可减轻SNI大鼠的机械性痛敏,抑制海马区LPCAT2和PAFR的表达,海马区PAF/PAFR信号通路可能参与了SNI大鼠神经病理性疼痛机制。
[Abstract]:Objective to investigate the role of platelet activating factor (PAF) synthase (LPCAT _ 1 / LPCAT _ 2) and its receptor (PAFR) in neuropathic pain induced by selective nerve injury (SNI) in rat sciatic nerve. Methods Forty male Sprague-Dawley rats with successful hippocampal tube placement were selected. The rats were randomly divided into 4 groups: sham group, model group, model ginkgolide group B BN52021 group, and solvent control group SMI HBC group. SNI BN52021 group was given Ginkgolide B10 渭 g through hippocampus 2 days after SNI operation. In 1 渭 L HBC solution, 1 渭 L HBC solution was given to the SNI HBC group. The mechanical claw threshold (PWMTT) and the latent period of thermal contraction of claw were recorded 1 day before operation and 1 day after operation, and the expression of LPCAT1, LPCAT2 and PAFR in fresh hippocampal tissue of rats were detected by RT-PCR on the 15th day. Results on the first day after operation, the PWMT value of SNI rats decreased significantly (P 0.05), the expression of LPCAT2 and PAFR increased (P 0.05) and the expression of LPCAT2 and PAFR in hippocampus decreased (P 0.05) in the group of BN52021 microinjection into the hippocampus, but the expression of LPCAT2 and PAFR in the hippocampus increased significantly (P 0.05), and the expression of LPCAT2 and PAFR in the hippocampus decreased in the group of BN52021 microinjection into the hippocampus (P 0.05). Conclusion the microinjection of ginkgolide B in hippocampus can reduce the mechanical pain sensitivity and inhibit the expression of LPCAT2 and PAFR in the hippocampus of SNI rats. The PAF/PAFR signal pathway in the hippocampus may be involved in the neuropathic pain mechanism of SNI rats.
【作者单位】：复旦大学附属浦东医院麻醉科;湖北医药学院附属太和医院疼痛科;上海市浦东新区周浦医院麻醉科;
【基金】：湖北省教育厅科研基金资助项目(No.D20132405) 上海市浦东新区卫生人才培养计划资助项目(No.PWRd2014-19)
【分类号】：R402

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1 杨京利;张力;向勇;马国平;;海马区血小板活化因子合酶及其受体的表达对选择性神经损伤大鼠痛觉敏化的影响[J];华中科技大学学报(医学版);2017年04期

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