基于免疫磁球的埃博拉病毒糖蛋白高灵敏比色检测

发布时间：2018-04-24 22:40

  本文选题：埃博拉病毒 + 磁球　； 参考：《分析科学学报》2017年05期

【摘要】：用免疫磁球捕获埃博拉病毒糖蛋白,与生物素化抗体形成免疫夹心复合物,然后链霉亲和素标记辣根过氧化物酶(SA-HRP)催化3,3',5,5'-四甲基联苯胺(TMB)进行显色,通过370 nm处吸光度对病毒糖蛋白进行定量。对免疫磁球进行了免疫荧光表征,通过对照实验验证方法的可靠性,并对检测条件进行了优化。结果表明,吸光度与病毒糖蛋白浓度在1.0~25.0 ng/m L内呈线性关系,检出限达到0.18 ng/m L。该方法重现性较好,特异性好,抗干扰能力强,可实现复杂样品中埃博拉病毒的检测。
[Abstract]:The Ebola virus glycoprotein was captured with the immunomagnetic ball, and the immunofluorescence complex was formed with the biotinylated antibody. Then the streptomycin labeled horseradish peroxidase (SA-HRP) catalyzed 3,3', 5,5'- four methylbiphenyl amine (TMB) to color and quantified the virus sugar protein through the absorbance at 370 nm. The immunomagnetic sphere was immunofluorescent table. The results showed that the absorbance and the concentration of virus glycoprotein in 1.0~25.0 ng/m L were linear, and the detection limit reached 0.18 ng/m L.. The results showed that the method had good reproducibility, good specificity and strong anti-interference energy, and the detection of Ebola virus in complex samples could be realized.

【作者单位】： 生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院;
【基金】：国家自然科学基金(No.21475099)
【分类号】：O657.3;R446.6
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本文编号：1798590