治疗中心静脉导管感染抗生素锁技术指标的量化研究

发布时间：2018-05-04 07:47

本文选题：抗生素锁 + 中心静脉导管　；参考：《中华医院感染学杂志》2017年20期

【摘要】：目的在使用抗生素锁(ALT)的常规药物浓度范围内和常用的锁定时间内,寻找灭菌效果最佳的药物浓度和锁定时间。方法采用置片法将含有细菌生物膜的硅胶薄膜样本随机分成300倍最低抑菌浓度(MIC)组、600倍MIC组、900倍MIC组、空白组等四组,各组5份样本,每组在6、12、18、24h四个时间点取出处理后,通过稀释平板计数法进行细菌计数定量培养,比较ALT最佳使用时间及浓度。结果不同浓度下最佳时间:300倍MIC组各时间点的细菌计数与空白对照组比较差异无统计学意义;600倍MIC组与对照组相比6h开始细菌计数明显下降(P0.05),12h与6h、18h与24h相比细菌计数无统计学差异,18h与6h、12h相比细菌计数有统计学差异(P均0.05);900倍MIC组与对照组比较6h时细菌计数显著下降(P0.05),6h与12h比较细菌计数出现显著性差异(P0.05),12h与18h、24h比较细菌计数无明显差异;不同时间下最有效浓度:在相同锁定时间内与对照组的细菌计数比较,300倍MIC组细菌计数无显著差异性,600倍MIC组和900倍MIC组细菌计数明显降低,差异性有统计学意义(P0.05);600倍MIC组和900倍MIC组之间细菌计数比较有显著性差异(P0.05)。结论 300倍MIC的实验用抗生素作为ALT溶液不能有效清除细菌生物膜,而600倍和900倍MIC的抗生素溶液均具有良好的清除细菌生物膜的作用;且在相同的锁定时间内,浓度越高,其清除生物膜的能力越强,效果越好;600倍MIC实验用抗生素作为ALT溶液,锁定时间18h效果最好;900倍MIC实验用抗生素作为ALT溶液,锁定12h效果最好。
[Abstract]:Objective to find out the best concentration and locking time of antibiotics in the range of routine drug concentration and the commonly used locking time. Methods the samples of silica gel film containing bacterial biofilm were randomly divided into three hundred times minimum inhibitory concentration (MIC) group, six hundred times MIC group, nine hundred times MIC group, one blank group and four groups. The optimal use time and concentration of ALT were compared by dilution plate counting method. Results there was no significant difference in bacterial count between the control group and the control group at the best time of different concentration: 300-fold MIC group. There was no significant difference between the MIC group and the control group. The number of bacteria began to decrease significantly in the MIC group compared with the control group at 6h, P0.05h 12h, 6h 18h and 24h compared with the control group. There was no significant difference in bacterial count between 18h and 6h, there was significant difference in bacterial count between MIC group and control group at 6h. There was no significant difference in bacterial count between 12h and 18h in MIC group compared with control group at 6h and 12h. There was no significant difference in bacterial count between 12h and 18h. The most effective concentration at different time: the bacterial count of 300-fold MIC group was not significantly different from that of control group in the same locking time. The bacterial count of 600-fold MIC group and 900-fold MIC group was significantly lower than that of control group. There was significant difference in bacterial count between the 600-fold MIC group and the 900-fold MIC group (P 0.05). Conclusion the bacteriological biofilm could not be effectively removed by using the antibiotic solution of 300-fold MIC as ALT solution, while the antibiotic solution of 600-fold and 900-fold MIC had a good effect on the removal of bacterial biofilm, and the higher the concentration was, the higher the concentration was in the same locking time. The stronger the ability of scavenging biofilm, the better the effect was, 600 times of MIC was used as ALT solution, and the locking time was 18 h. The best effect was 900 times of MIC as ALT solution, and 12 h as the best.
【作者单位】：长江大学医学院护理系;新疆医科大学第一附属医院重症监护病房;长江大学医学院基础学部;长江大学医学院实验中心;
【基金】：湖北省卫生和计划生育委员会基金资助项目(WJ2015HB036)
【分类号】：R472

【相似文献】