透析机与供水回路间软管生物膜形成对透析用水微生物指标的影响分析

发布时间：2018-05-06 03:34

本文选题：透析用水 + 薄膜过滤法　；参考：《中华医院感染学杂志》2017年18期

【摘要】：目的观察执行血液透析及相关治疗用水(YY0572-2015)标准后,透析用水微生物培养结果的变化,分析水路污染的风险因素。方法依照YY0572-2015要求,采集2017年1月-6月供水回路反渗水回水口、出水口的透析用水各10份,同时采集透析机与供水回路软管连接处的透析用水128份,薄膜过滤法富集后,用TGEA培养皿进行微生物培养,取不同使用年限软管内壁行电镜扫描。结果供水回路反渗水回水口、出水口的透析用水微生物培养结果均为阴性;透析机与供水回路软管连接处的透析用水128份均有菌落生长,其中革兰阴性菌较多占60.16%;4组使用不同年限软管的透析用水细菌数量构成不同,并且电镜下软管内壁存在生物膜结构。结论透析机与供水回路间连接软管检出微生物以革兰阴性菌为主,随着软管使用时间的延长,细菌菌落呈现增长趋势,软管内壁产生生物膜,是污染透析用水的主要因素,可采用U型回路连接管或者定期维护更换软管等措施消除风险。
[Abstract]:Objective to observe the changes of microbial culture results of dialysis water after implementing the standard YY0572-2015, and to analyze the risk factors of water pollution. Methods according to the requirement of YY0572-2015, the reverse seepage water outlet of the water supply circuit was collected from January to June 2017, and the dialysis water from the outlet was 10 parts each. At the same time, the dialysis water was collected from the connection between the dialyzer and the hose of the water supply circuit. The microorganism was cultured in TGEA culture dish, and the inner wall of hose was scanned by electron microscope with different service life. Results the microorganism culture results of dialysis water were negative in the reverse seepage outlet of water supply circuit, and the colony growth was found in 128 parts of dialysis water used in connection between dialyzer and hose of water supply circuit. Gram-negative bacteria accounted for 60.16% of the total number of bacteria in dialysis water used for different years, and there was biofilm structure in the inner wall of hose under electron microscope. Conclusion Gram-negative bacteria were the main microorganism detected in the connecting hose between dialyzer and water supply loop. With the extension of hose service time, bacterial colony showed an increasing trend, and biofilm was produced in the inner wall of hose, which was the main factor to pollute dialysis water. U-loop connecting pipe or regular maintenance and replacement hose can be used to eliminate risks.
【作者单位】：青海大学附属医院医院感染管理科;
【分类号】：R459.5

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