双歧杆菌对中老年人健康影响的菌种特异性初探

发布时间：2018-05-17 11:26

本文选题：中老年人 + 双歧杆菌　；参考：《四川大学学报(医学版)》2017年05期

【摘要】：目的初探双歧杆菌对中老年人健康影响的菌种特异性。方法纳入成都地区148例50~75岁健康中老年人,收集其血液和粪便标本,测定血液生化和免疫指标,使用细菌的16SrRNA基因序列设计双歧杆菌属及种的特异性引物,应用实时荧光定量PCR对受试中老年人粪便中的双歧杆菌属及其人体最常见的8个菌种进行定性定量分析。结果受试中老年人粪便中均可检出双歧杆菌,菌量约为108个/g湿便,检出率为100%,且可检出通常被认为是婴幼儿肠道内的优势双歧杆菌菌种。相关性分析结果显示,双歧杆菌属与尿素氮呈正相关(r=0.214,P0.05);青春双歧杆菌和体质量指数呈负相关(r=-0.311,P0.05),链双歧杆菌与其呈正相关(r=0.167,P0.05);高密度脂蛋白胆固醇和短双歧杆菌呈负相关(r=-0.247,P0.05),与婴儿双歧杆菌呈正相关(r=0.350,P0.05);两歧双歧杆菌与免疫球蛋白IgA呈正相关(r=0.365,P0.05)。结论肠道双歧杆菌能菌种特异性地影响机体的生理代谢和免疫机能,保持肠道双歧杆菌菌种正常的组成可能是减缓高龄引起的免疫衰退、维持中老年人健康的有效方法之一。
[Abstract]:Objective to explore the strain specificity of Bifidobacterium on the health of middle and old people. Methods 148 healthy adults aged 50 to 75 years old in Chengdu were included. Blood and fecal samples were collected, blood biochemical and immune indexes were determined, and specific primers of Bifidobacterium and species were designed using 16SrRNA gene sequence of bacteria. The bifidobacterium and its eight most common strains in feces of middle and old people were analyzed qualitatively and quantitatively by real-time fluorescence quantitative PCR. Results Bifidobacterium could be detected in feces of middle and old people, the amount of bacteria was about 108 / g wet stool, the detection rate was 100, and it could be found that bifidobacterium was considered to be the dominant strain of bifidobacterium in the intestinal tract of infants and young children. The results of correlation analysis show that Bifidobacterium has positive correlation with urea nitrogen, bifidobacterium is negatively correlated with body mass index (BMI), Streptococcus bifidobacterium is positively correlated with urea nitrogen, high density lipoprotein cholesterol and Bifidobacterium brevis are negatively correlated with Bifidobacterium infantile bifidobacterium. There was a positive correlation between Bifidobacterium bifidobacterium and immunoglobulin IgA. Conclusion Bifidobacterium can specifically affect the physiological metabolism and immune function of the body. Keeping the normal composition of bifidobacterium in the intestinal tract may be one of the effective methods to slow down the immune decline caused by old age and to maintain the health of the middle-aged and the elderly.
【作者单位】：四川大学华西公共卫生学院营养食品卫生与毒理学系;河北医科大学公共卫生学院卫生检验教研室;四川大学华西公共卫生学院卫生检验与检疫系;
【基金】：达能营养基金(No.14H0983)资助
【分类号】：R446

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