122例直接抗人球蛋白试验阳性病例分析

发布时间：2018-05-25 09:15

本文选题：直接抗人球蛋白试验 + 抗体分型　；参考：《免疫学杂志》2017年05期

【摘要】：目的了解直接抗人球蛋白试验(direct antiglobulin test,DAT)阳性在临床输血患者中的分布,并分析不同疾病DAT抗体和补体类型。方法采用微柱凝胶抗人球蛋白卡对拟输血患者进行输血前检测,DAT阳性标本BIO-RAD抗体分型卡作抗体及补体类型鉴别。结果 4 724例标本中,筛选出122例DAT阳性患者,阳性率为2.58%。在122例DAT阳性标本中,血液系统疾病占41.8%,其次为新生儿溶血病(hemolytic disease of newborn,HDN)和感染性疾病,分别占14.75%和10.66%。DAT抗体及补体分型鉴定结果显示单纯IgG阳性率最高,占76.22%,其次为IgG/C3d、IgG/IgM/C3d和C3d,阳性率分别为9.84%、4.10%和3.28%。DAT抗体在疾病中的分布以IgG为主,但在自身免疫性溶血性贫血(autoimmune hemolytic anemia,AIHA)及系统性红斑狼疮(systemic lupus erythematosus,SLE)患者中,抗体种类包括IgG、IgA和IgM,补体包括C3d和C3c两种。结论 DAT阳性标本中,血液系统疾病所占比率最大,其抗体类型在临床疾病中以单纯IgG阳性为主,而在SLE和AIHA患者中却含有多种抗体和补体。
[Abstract]:Objective to investigate the distribution of direct antiglobulin test (DAT) positive in clinical transfusion patients, and to analyze the DAT antibody and complement types in different diseases. Methods microcolumn gel anti-human globulin card was used to identify the BIO-RAD antibody typing card and complement type in the blood-imitated patients before blood transfusion. Results among 4 724 specimens, 122 DAT positive cases were screened, the positive rate was 2.58%. Hemolytic disease of newborn hemolytic disease of newbornus disease (14.75%) and 10.66%.DAT antibody and complement typing showed the highest positive rate of IgG in 122cases of DAT positive specimens, 41.8% were hemolytic disease of newbornus, and 14.75% were infective diseases, respectively, and the results of 10.66%.DAT antibody and complement typing showed that the positive rate of IgG alone was the highest. The positive rates were 9.84% and 4.10%, respectively, and the distribution of 3.28%.DAT antibodies in the disease was mainly IgG, but in the patients with autoimmune hemolytic anemia (AA) and systemic lupus erythematosus (systemic lupus erythematosus), the positive rates were 9.84% and 4.10%, respectively, but in the patients with autoimmune hemolytic anemia (AA) and systemic lupus erythematosus (SLE), the positive rates were 9.84% and 4.10%, respectively. The types of antibodies included IgGA and IgM, and the complement consisted of C 3 d and C 3 c. Conclusion among the DAT positive specimens, the proportion of hematological diseases is the largest. The type of antibodies is mainly IgG positive in clinical diseases, but there are many kinds of antibodies and complements in SLE and AIHA patients.
【作者单位】：中南大学湘雅医院输血科;江西省萍乡市湘雅萍矿合作医院输血科;中国人民解放军空军航空医学研究所附属医院检验科;
【基金】：湖南省自然科学基金(11JJ6072)
【分类号】：R457.1

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