西安地区献血人群MNS血型基因多态性研究

发布时间：2018-05-31 23:16

本文选题：MNS血型 + 献血者　；参考：《中国输血杂志》2017年06期

【摘要】：目的了解西安地区人群MNS血型基因的多态性。方法随机选取陕西西安地区献血人群200(人)份血样,以血型血清学鉴定其MN和Ss表现型;同时提取全血中的DNA,对血型糖蛋白相关基因GYPA的内含子(intron)-2、3及外显子(exon)-2与GYPB的exon-3(B4)序列扩增后测序;分析本组人群MNS等位基因的多态性并与不同地区人群的M基因各型的构成比作比较。结果本组人群的MN、Ss血型血清学定型与基因测序分型结果完全一致,其中M、N、S、s基因频率分别为0.467、0.533、0.053和0.947,MG、MT基因频率分别为0.415和0.052;本组人群M基因各型的构成比与北京和深圳人群不同;GYPA intron-2的第59与60位碱基处插入T,发生率为20.5%(41/200)。结论西安地区献血人群MNS血型基因具有多态性,对这种多态性的了解有助于本地区临床科学、合理输血以及开展人类群体遗传学特征及分子生物学的研究。
[Abstract]:Objective to investigate the polymorphism of MNS blood group gene in Xi'an population. Methods 200 (human) blood samples of blood donors from Xi'an area of Shaanxi Province were randomly selected and their MN and Ss phenotypes were identified by blood type serology. DNA was extracted from whole blood at the same time, the intron of GYPA and exonon-2 of exon 2 and exon-3 of GYPB were amplified and sequenced. The polymorphism of MNS allele was analyzed and compared with that of M gene in different regions. Results the serological typing of MNN Ss blood group was consistent with the results of gene sequencing. Among them, the frequencies of intron-2 gene were 0.415 and 0.052, respectively, and the frequencies of M gene were 0.415 and 0.052, respectively. The ratio of the composition of M gene in this group was different from that in Beijing and Shenzhen population at the 59th and 60th sites of GYPA intron-2, and the incidence rate was 20.551 / 2000.The results showed that the frequency of M gene was 0.415 and 0.052 respectively, and the incidence of T insertion was 20.51p / 2000.The frequency of M gene was different from that of Beijing and Shenzhen population at the 59th and 60th sites of GYPA gene. Conclusion the polymorphism of MNS blood group gene in blood donors in Xi'an is helpful to clinical science, reasonable blood transfusion and the study of human population genetics and molecular biology.
【作者单位】：西安交通大学医学部基础医学院病原生物学与免疫学系;西安市卫生学校;深圳市血液中心;陕西省血液中心;
【基金】：西安市卫生和计划生育委员会资助项目(J2014051)
【分类号】：R446.6

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