利用探针跳跃式离子电导显微镜研究活体血小板膜结构及其膜微粒的形成

发布时间：2018-06-05 13:41

本文选题：探针跳跃式离子电导显微镜 + 血小板活化　；参考：《生物医学工程学杂志》2017年05期

【摘要】：血小板在激活剂作用下会快速活化并释放具有高促凝活性的血小板膜微粒(PMPs),进而诱发出凝血功能障碍,但PMPs的形成机制有待明确。探针跳跃式离子电导显微镜(HPICM)具有在生理环境下对活体细胞进行非接触式实时高分辨率成像的技术优势。本文运用HPICM技术实时监测血小板在胞内钙离子诱导剂A23187和细胞松弛素D作用下活化及PMPs形成的过程,证明了胞内钙离子浓度和细胞骨架蛋白在血小板活化及PMPs形成中发挥了重要作用;并发现与扁平型血小板相比,突起型血小板对A23187和细胞松弛素D更为敏感,这对利用HPICM技术研究血小板活化与出凝血功能调节之间的关系具有指导意义。
[Abstract]:Platelets can be activated and released rapidly under the action of activator, which can induce coagulation dysfunction. However, the mechanism of PMPs formation needs to be clarified. Probe jump ion conductance microscope (HPICM) has the advantage of non-contact real-time high-resolution imaging of living cells in physiological environment. The process of platelet activation and PMPs formation under the action of intracellular calcium inducer A23187 and cytochalasin D was monitored by HPICM technique. The results showed that intracellular calcium concentration and cytoskeleton protein played an important role in platelet activation and PMPs formation, and that protruded platelets were more sensitive to A23187 and cytochalasin D than platelets with flat platelets. It is helpful to study the relationship between platelet activation and coagulation regulation by HPICM.
【作者单位】：国家纳米技术与工程研究院;天津市永久医院;天津医科大学总医院神经外科;天津市神经病学研究所;
【基金】：天津市科技计划(14ZCZDSY00020) 天津市滨海新区塘沽科技兴区项目(2012XQ15-11) 国家自然科学基金青年科学基金项目(31300828)
【分类号】：R446.1

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