基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法

发布时间：2018-06-07 00:09

本文选题：壳层隔绝纳米粒子 + 氰化物　；参考：《分析化学》2017年05期

【摘要】：建立了一种快速、简便的基于壳层隔绝纳米粒子和在线裂解捕集技术的血液氰化物表面增强拉曼光谱(SERS)检测方法。构建了新型在线裂解-吹扫捕集装置,通过浓H_2SO_4酸化,将全血中结合态氰化物裂解,并转化为挥发性的HCN气体,经N_2吹扫至碱液中形成NaCN,实现了中毒血液中氰化物的高效裂解与捕集;在强碱性条件下,以壳层隔绝金纳米粒子为基底,极大地削弱了氰化物的溶金效应,使得检测信号稳定,存在时间由普通金纳米粒子的5 min提高至1 h;方法检出限达10μg/L,线性范围为100~2000μg/L。将此方法应用于氰化物染毒大鼠及真实氰化物中毒患者全血的快速检测,为氰化物中毒快速检测提供了一种灵敏、特异、可靠的新方法。
[Abstract]:A rapid, simple and simple method of detection of blood cyanide surface enhanced Raman spectroscopy (SERS) based on shell isolation nanoparticles and on-line cracking capture technology was established. A new on-line pyrolysis blowing and trapping device was constructed. Through strong H_2SO_4 acidification, the combined cyanide in the whole blood was cracked and converted to volatile HCN gas by N_2 blowing. NaCN was formed in the alkaline solution to achieve the efficient lysis and capture of cyanide in the poisoned blood. Under the strong alkaline condition, the gold nanoparticles were isolated from the shell, which greatly weakened the gold dissolution effect of cyanide. The detection signal was stable and the existence time was increased from 5 min to 1 h, and the detection limit was 10 g/L, The linear range of 100~2000 mu g/L. is applied to the rapid detection of whole blood in cyanide infected rats and patients with real cyanide poisoning. It provides a sensitive, specific and reliable new method for the rapid detection of cyanide poisoning.
【作者单位】：军事医学科学院毒物药物研究所抗毒药物与毒理学国家重点实验室;军事医学科学院附属医院中毒救治科;
【基金】：国家重大科学仪器设备开发专项(No.2011YQ03012411) 重大新药创新国家科技重大专项"中药新药安全性检测技术与标准研究"项目(No.2014ZX09304307001)资助~~
【分类号】：O657.37;R446.1

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