严重失血性休克大鼠液体复苏后的免疫修复（英文）

发布时间：2018-06-07 23:56

本文选题：调节性T细胞 + 辅助性T细胞　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年05期

【摘要】：目的:严重失血性休克大鼠模型早期阶段使用不同的液体复苏,比较脾脏组织中调节性T细胞(Tregs)、辅助性T细胞1(Th1)/Th2以及细胞毒性T细胞1(Tc1)/Tc2的不同变化,初步探讨其免疫修复机制。创新点:(1)脾脏为机体重要免疫器官,检测其中的免疫细胞变化,比外周血更具敏感性和特异性;(2)将免疫反应中多环节的免疫细胞变化进行协同分析,结果更具创新性和科学性,为临床上形成规范的救治方案提供了科学的实践资料。方法:将SD雄性大鼠随机分成5组,其中对照组和Sham组(假手术)仅作为比较,其余三组在建立严重失血性休克大鼠模型后,采用不同的液体复苏:等渗盐水(NS组)、高渗盐水(HTS组)和羟乙基淀粉(HES组)。然后再灌注30分钟,并持续监测血液动力学120分钟,最后心脏穿刺,取脾脏组织,通过三色荧光标记流式细胞术进一步分析CD4~+CD25~+Foxp3~+Treg细胞含量,以及Th1/Th2和Tc1/Tc2的比值。结论:液体复苏后大鼠脾脏中CD4~+CD25~+Foxp3~+Tregs细胞含量、Th1/Th2和Tc1/Tc2的比值在对照组、Sham组和HTS组中无差异,并都显著高于NS组和HES组。与Sham组比较,HTS组中Tc1水平明显升高,而NS组、HES组和HTS组中Tc2水平均有升高,且三组之间Tc2水平无差别。因此,对于维持脾脏中Treg细胞含量、Th1/Th2和Tc1/Tc2平衡的作用上,HTS液体复苏对免疫系统的影响大于NS和HES。综上所述,在失血性休克后的早期阶段HTS复苏可提供潜在的免疫修复作用。
[Abstract]:Objective: to compare the effects of different fluid resuscitation in the early stage of severe hemorrhagic shock on the changes of regulatory T cell Tregsn, helper T cell 1T 1 / Th2 and cytotoxic T cell 1Tc 1 / Tc 2 in spleen tissue, and to explore the mechanism of immune repair. The spleen is an important immune organ of the body. The changes of immune cells in the spleen are more sensitive and specific than that in the peripheral blood. (2) the changes of the immune cells in multiple links in the immune response are analyzed collaboratively, and the results are more innovative and scientific. It provides scientific and practical data for the establishment of standardized treatment plan in clinical practice. Methods: Sprague-Dawley male rats were randomly divided into five groups. The control group and Sham group (sham-operated group) were compared only. The other three groups were treated with severe hemorrhagic shock model. Different kinds of fluid resuscitation were used: isosmotic saline group NS group, hypertonic saline solution group (HTS group) and hydroxyethyl starch group (HES group). After 30 minutes of reperfusion, the hemodynamics was continuously monitored for 120 minutes. At last, the heart was punctured and the spleen tissue was taken. The content of CD4 ~ CD25 ~ Foxp3 ~ Treg cells and the ratio of Th1 / Th2 to Tc1 / Tc2 were further analyzed by flow cytometry. Conclusion: the ratio of Th1 / Th2 and Tc1 / Tc2 in spleen of rats after fluid resuscitation was significantly higher than that of NS and HES groups, and the ratio of Th1 / Th2 and Tc1 / Tc2 in spleen of rats after fluid resuscitation was significantly higher than that of NS and HES groups. The level of Tc1 in HTS group was significantly higher than that in Sham group, but Tc2 level in HES group and HTS group was increased, and there was no difference in Tc2 level among the three groups. Therefore, the effects of HTS fluid resuscitation on the immune system in maintaining the balance of Th1 / Th2 and Tc1 / Tc2 of Treg cells in spleen were greater than those of NS and HES. In conclusion, HTS resuscitation in the early stage of hemorrhagic shock can provide potential immune repair.
【作者单位】： Department
【基金】：supported by the National Natural Science Foundation of China(No.81272075)
【分类号】：R459.7

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