AAPBA@KCC-1的合成及其对血清中唾液酸和神经节苷脂的分析应用

发布时间：2018-06-08 15:54

本文选题：分散固相萃取 + 介孔二氧化硅KCC-　；参考：《色谱》2017年05期

【摘要】：将3-丙烯酰胺基苯硼酸(AAPBA)修饰在介孔二氧化硅KCC-1的表面,合成AAPBA@KCC-1材料,并通过扫描电子显微镜、透射电子显微镜、N_2吸附解吸附测试和X射线光电子能谱对其进行表征;将AAPBA@KCC-1材料作为分散固相萃取(dSPE)的吸附剂,对血清样品中的唾液酸和神经节苷脂(GLS)进行提取和富集;采用傅里叶变换离子回旋共振质谱法和超高效液相色谱-离子淌度-四极杆飞行时间质谱法分别对AAPBA@KCC-1材料吸附得到的唾液酸和神经节苷脂进行分析。结果表明,AAPBA@KCC-1材料能够有效吸附唾液酸和神经节苷脂,简化了样品前处理的操作,可以进一步应用于生物样品中唾液酸和神经节苷脂的研究。
[Abstract]:AAPBA was modified on the surface of mesoporous silica KCC-1. AAPBA-kCC-1 was synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), absorption desorption test (TEM) and X-ray photoelectron spectroscopy (XPS). The sialic acid and ganglioside GLSs in serum samples were extracted and enriched by using AAPBABA-KCC-1 as adsorbent for dispersive solid-phase extraction (SPE). The sialic acid and ganglioside adsorbed by AAPBAR KCC-1 material were analyzed by Fourier transform ion cyclotron resonance mass spectrometry and ultra-high performance liquid chromatography-ion mobility quadrupole time-of-flight mass spectrometry respectively. The results showed that AAPBABALKCC-1 could effectively adsorb sialic acid and ganglioside, simplify the operation of sample pretreatment, and could be further applied to the study of sialic acid and ganglioside in biological samples.
【作者单位】：上海交通大学药学院;
【基金】：上海市科委科研计划项目(15142200200,16142200300) 中国博士后科学基金(2015M581628) 上海市引进技术的吸收与创新专项(XC-ZXSJ-02-2016-11)~~
【分类号】：O647.33;R446.1

【相似文献】