标本类型和离心时间对临床常用心肌损伤标志物检测结果的影响

发布时间：2018-06-16 13:56

本文选题：肝素锂 + 乙二胺四乙酸二钾　；参考：《检验医学与临床》2017年08期

【摘要】：目的探讨肝素锂抗凝血浆、乙二胺四乙酸二钾(EDTA-K2)抗凝血浆、血清等3类标本和缩短离心时间对肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)和肌红蛋白(Myo)等心肌损伤标志物检测结果的影响。方法使用VITROS 5600生化免疫分析仪检测28例3类标本的心肌损伤标志物,并进一步行cTnI的EDTA-K2干扰试验;将21例肝素锂抗凝标本在4 000r/min分别离心2和10min后检测心肌损伤标志物。采用SigmaPlot 11.0统计软件进行分析。结果 EDTA-K2抗凝组cTnI比肝素锂抗凝组低约15%,血清组CK-MB比肝素锂抗凝组高出大概7%,差异均有统计学意义(P0.05)。干扰试验证实EDTA-K2对检测cTnI存在负干扰。将肝素锂抗凝标本离心2min与10min的心肌损伤标志物比较,差异无统计学意义(P0.05)。结论肝素锂抗凝血浆、EDTA-K2抗凝血浆和血清的心肌损伤标志物检测结果间存在不一致性;缩短离心时间既不影响检测结果,又能缩短标本周转时间,使临床和患者获益。
[Abstract]:Objective to investigate the effects of heparin lithium anticoagulant plasma, EDTA-K2) anticoagulant plasma, serum and shortened centrifugation time on the detection of cardiac damage markers such as cardiac troponin I ~ (cTnI), creatine kinase isoenzyme CK-MBand myoglobin. Methods VITROS 5600 biochemical immunoassay was used to detect myocardial damage markers in 28 cases of 3 kinds of specimens, and EDTA-K2 interference test of cTnI was performed, and 21 cases of heparin lithium anticoagulant specimens were centrifuged 2 and 10min respectively at 4 000r/min to detect myocardial damage markers. SigmaPlot 11.0 statistical software was used to analyze. Results the cTnI in EDTA-K2 anticoagulant group was about 15% lower than that in heparin lithium anticoagulant group, and CK-MB in serum group was about 7% higher than that in heparin lithium anticoagulant group. The difference was statistically significant (P 0.05). The interference test confirmed that EDTA-K2 had negative interference on the detection of cTnI. There was no significant difference in myocardial injury markers between centrifuged 2min and 10min in heparin lithium anticoagulant specimens. Conclusion there is inconsistency between the detection results of myocardial injury markers in anticoagulant plasma EDTA-K2 and serum in heparin lithium anticoagulant plasma, and shortening the centrifugation time does not affect the test results, but also shortens the sample turnover time, which is beneficial to both clinical and patients.
【作者单位】：江苏省南通大学附属医院检验医学中心;
【基金】：国家自然科学基金资助项目(81201349) 江苏省南通市前沿与关键技术创新项目(MS22015049)
【分类号】：R542.2;R446.1

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