D-酪氨酸联合万古霉素对体外耐甲氧西林金黄色葡萄球菌及生物膜的消除作用研究

发布时间：2018-06-19 13:37

本文选题：耐甲氧西林金黄色葡萄球菌 + 细菌生物膜　；参考：《中华医院感染学杂志》2017年22期

【摘要】：目的观察D-酪氨酸(D-tyrosine)联合万古霉素对体外培养耐甲氧西林金黄色葡萄球菌(MRSA)及其生物膜的消除作用,旨在为假体周围感染(PJI)的临床治疗提供可行性依据。方法导片法建立体外MRSA生物膜模型,将实验分为空白组,D-酪氨酸组,万古霉素组,联合组(D-酪氨酸+万古霉素),每组干预1h、6h、12h、24h后分别用结晶紫及荧光染色,分别在高倍镜及激光共聚焦显微镜(CLSM)下观察不同组MRSA及其生物膜的变化情况。结果除空白组外其他组中细菌密度随时间增加而降低,D-酪氨酸和联合组明显,每组之间均有统计学差异;12小时内万古霉素组及联合组中死菌比例会随着时间增加而增加,且联合组与万古霉素组比较有统计学意义(P0.05),12小时后万古霉素组死菌比例无明显变化,联合组死菌比例进一步增加。结论D-酪氨酸对体外培养MRSA生物膜具有分散作用;D-酪氨酸联合万古霉素对生物膜中的MRSA具有更好的消除作用。
[Abstract]:Objective to observe the effect of D tyrosine D tyrosine combined with vancomycin on the elimination of methicillin resistant Staphylococcus aureus (MRSA) and its biofilm in vitro in order to provide a feasible basis for the clinical treatment of periprosthetic infection (PJI). Methods the model of MRSA biofilm in vitro was established by guided tablet method. The experiment was divided into three groups: blank group (D tyrosine group), vancomycin group (group C) and combined group (group D tyrosine vancomycin). Each group was treated with crystal violet and fluorescence staining after 1 hour intervention for 6 h and 12 h for 24 h. The changes of MRSA and biofilm in different groups were observed under high power microscope and laser confocal microscope (CLSM) respectively. Results the bacterial density in the other groups except the blank group decreased significantly with the increase of time, and there was a significant difference between the two groups. The percentage of dead bacteria in vancomycin group and combined group increased with time within 12 hours. Compared with vancomycin group, there was no significant difference in the proportion of dead bacteria between the combined group and vancomycin group after 12 hours, but the proportion of dead bacteria in the combined group was further increased. Conclusion D- tyrosine can disperse MRSA biofilm in vitro. D- tyrosine combined with vancomycin can eliminate MRSA in biofilm.
【作者单位】：广州医科大学附属广州市第一人民医院骨关节外科;遵义医学院第五附属珠海医院骨二科;
【基金】：广州市科技计划基金资助项目(2014KP000075)
【分类号】：R446.5

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