人类白细胞抗原A、B和DRB1测序分型模棱两可结果的分布及解决

发布时间：2018-06-19 22:01

本文选题：人类白细胞抗原 + 模棱两可　；参考：《重庆医学》2017年13期

【摘要】：目的调查广西人群HLA-A、B、DRB1基因测序分型中模棱两可结果的分布状况,提出解决方案。方法采用聚合酶链反应-直接测序分型方法对1 000名中华骨髓库广西分库供者的HLA-A、B、DRB1基因进行测序分型,分析3个基因座模棱两可分型结果的分布情况,并分别采用高分辨聚合酶链反应-序列特异性引物法和组特异性测序引物法进行解决。结果 1 000份标本中,96.7%的标本HLA-A、B、DRB1基因至少有1个位点出现模棱两可结果,其中HLA-A、B、DRB1各位点出现模棱两可结果的比例分别为65.7%、58.8%、77.2%。对于检出的模棱两可结果标本,单独采用组特异性测序引物法可以解决87.37%HLA-A、93.54%HLA-B和60.49%的HLA-DRB1基因模棱两可分型结果,使用高分辨聚合酶链反应-序列特异性引物法解决12.63%HLA-A、4.76%HLA-B和15.29%HLA-DRB1基因模棱两可分型结果,剩余1.70%HLA-B和24.22%的HLA-DRB1基因模棱两可分型结果联合使用组特异性测序引物法和高分辨聚合酶链反应-序列特异性引物法解决。结论组特异性测序引物法和高分辨聚合酶链反应-序列特异性引物法分别对位于HLA-A、B和HLA-DRB1基因检测区内、外的模棱两可分型结果具有较高的解决能力,二者互为补充,可有效解决HLA高分辨基因分型结果模棱两可问题。
[Abstract]:Objective to investigate the distribution of ambiguous results in the sequencing typing of DRB1 alleles of HLA-AHG BV in Guangxi population, and to propose a solution. Methods Polymerase chain reaction (PCR) -direct sequencing typing method was used to analyze the distribution of ambiguous genotyping results of three loci in 1 000 Chinese bone marrow bank donors from Guangxi province. High resolution polymerase chain reaction-sequence specific primer method and group specific sequencing primer method were used to solve the problem. Results at least one of the 1 000 samples showed ambiguous results at least one locus of HLA-Ah-Bnb DRB1 gene, and the percentage of ambiguous results was 65.7%, 58.8% and 77.2%, respectively. For the detected ambiguous result specimens, using group specific sequencing primer method alone can solve 87.37 HLA-An 93.54B and 60.49% HLA-DRB1 genotyping results. High resolution polymerase chain reaction-sequence specific primer method was used to solve the results of 12.63 HLA-An 4.76 HLA-B and 15.29HLA-DRB1 genotyping. The remaining 1.70% HLA-B and 24.22% of HLA-DRB1 gene ambiguous typing results were solved by combination of group specific sequencing primer method and high resolution polymerase chain reaction-sequence specific primer method. Conclusion Group specific sequencing primer method and high resolution polymerase chain reaction-sequence specific primer method have a high ability to solve the ambiguous typing results of HLA-Amb gene and HLA-DRB1 gene, respectively, which are complementary to each other. It can effectively solve the ambiguity of HLA high resolution genotyping results.
【作者单位】：广西壮族自治区南宁中心血站/南宁输血医学研究所;
【基金】：广西壮族自治区南宁市科学研究与技术开发计划项目(201003048C-3) 广西壮族自治区卫生厅科技研究计划项目(Z2010198)
【分类号】：R440

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