不同类型蛋白胨支持大肠菌群生长效果评价

发布时间：2018-06-25 14:52

本文选题：大肠菌群 + 浊度测量　；参考：《中国公共卫生》2017年05期

【摘要】：目的快速、准确评价不同类型蛋白胨支持大肠菌群的生长效果,为优化大肠菌群快速生长培养基选择适宜原材料。方法选择不同类型的蛋白胨和大肠菌群代表性菌株,应用全自动生长曲线测定仪进行浊度测量,用原材料再配制培养基,通过测定培养基支持大肠菌群的生长能力验证原材料筛选方法的可靠性。结果多价蛋白胨支持大肠菌群生长能力较好;大肠菌群快速生长的蛋白胨评估与配制培养基后的实验结果一致,即培养24 h后最大A值均为1.18。结论不同类型蛋白胨支持大肠菌群的生长能力存在差异,生长曲线测定仪可作为培养基原材料选择的有力工具。
[Abstract]:Objective to evaluate the effect of different peptone on the growth of coliform bacteria, and to select suitable raw materials for optimizing the rapid growth medium of coliform bacteria. Methods different kinds of representative strains of peptone and coliform bacteria were selected. The turbidity was measured by automatic growth curve tester and the medium was prepared with raw materials. The reliability of raw material screening method was verified by measuring the growth ability of coliform bacteria supported by culture medium. Results the growth ability of coliform bacteria supported by polyvalent peptone was good, and the peptone evaluation of coliform group rapid growth was consistent with the experimental results after the preparation of culture medium, that is, the maximum A value of coliform bacteria was 1.18 after 24 h culture. Conclusion the growth ability of coliform bacteria supported by different types of peptone is different, and the growth curve tester can be used as a powerful tool for selecting the raw materials of culture medium.
【作者单位】：中国检验检疫科学研究院;北京奥博星生物技术有限责任公司;
【基金】：国家“十二五”科技支撑计划课题(2012BAD29B02) 国家质检总局科技计划项目(2012IK197)
【分类号】：R446.5

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