联合氢气吸入的液体复苏方案对脓毒性休克大鼠肾脏的保护作用

发布时间：2018-07-28 06:59

【摘要】：目的:评价联合2%氢气吸入的液体复苏方案对脂多糖(LPS)诱导的脓毒性休克大鼠肾脏的治疗效果。方法:60只Wistar大鼠随机分成4组(每组15只):正常对照(control)组,脓毒性休克(shock)组,脓毒性休克+液体复苏组(fluid组),脓毒性休克+2%氢气吸入联合液体复苏组(fluid+H_2组)。大鼠麻醉后予呼吸机辅助通气,fluid+H_2组给予2%氢气空气混合气,其余3组给予单纯空气吸入。10 mg/kg LPS静脉注射,建立脓毒性休克模型,control组给予等量生理盐水静脉注射。Fluid组和fluid+H_2组给予相同液体复苏方案,维持平均动脉压于正常水平。4 h后,大鼠经腹主动脉放血处死,留取血液及肾脏标本。结果:联合2%氢气吸入的液体复苏方案与单纯液体复苏比较,血肌酐、尿素氮、中性粒细胞明胶酶相关脂质运载蛋白及肾脏组织TNF-α、IL-6水平明显降低,氧化应激损伤程度明显减轻。结论:联合2%氢气吸入的液体复苏方案更能减轻脓毒症所致急性肾损伤。
[Abstract]:Aim: to evaluate the effect of fluid resuscitation combined with 2% hydrogen inhalation on the kidney of septic shock rats induced by lipopolysaccharide (LPS). Methods Sixty Wistar rats were randomly divided into 4 groups (15 rats in each group): normal control (control) group, septic shock (shock) group, septic shock fluid resuscitation group (fluid group), septic shock 2% hydrogen inhalation combined with liquid resuscitation group (fluid Hast2 group). After anesthesia, the rats were given 2% hydrogen and air mixture after ventilator assisted ventilation (H2) group, and the other three groups were injected intravenously with air inhalation for 10 mg/kg LPS. The septic shock model control group was given the same fluid resuscitation regimen by intravenous injection of normal saline. Fluid group and fluid Hast2 group were given the same fluid resuscitation regimen. After maintaining the mean arterial pressure at normal level for 4 h, the rats were bled out through the abdominal aorta. Blood and kidney samples were collected. Results: the levels of serum creatinine, urea nitrogen, neutrophil gelatinase-associated lipid carrier protein and TNF- 伪 IL-6 in renal tissue were significantly decreased and the degree of oxidative stress was significantly reduced in the fluid resuscitation regimen combined with 2% hydrogen inhalation as compared with that in the pure fluid resuscitation. Conclusion: fluid resuscitation combined with 2% hydrogen inhalation can reduce acute renal injury caused by sepsis.
【作者单位】：中国医科大学附属第一医院急诊科;中国医科大学附属盛京医院泌尿外科;
【基金】：国家自然科学基金资助项目(No.81571882)
【分类号】：R459.7

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