化学发光免疫分析技术在微生物检测中的应用

发布时间：2018-08-24 10:06

【摘要】：化学发光免疫分析技术凭借其化学发光的高灵敏性和免疫反应的高特异性在微生物快速检测中广泛应用。该文着重叙述了化学发光免疫分析技术核心检测体系在微生物检测中的研究进展,并对其涉及到的样品前处理和检测新方法进行了综述。化学发光免疫分析技术检测微生物用时短、成本低,但特异性识别方法和检测的灵敏度有待提升,新型的发光体系、发光放大方法、可替代抗体的识别分子以及相关的前处理技术是未来研究的重点。
[Abstract]:Chemiluminescence immunoassay (CLIA) is widely used in the rapid detection of microorganisms with its high chemiluminescence sensitivity and high specificity of immunoreaction. In this paper, the research progress of the core detection system of chemiluminescence immunoassay in microbial detection was reviewed, and the new methods of sample pretreatment and detection were reviewed. Chemiluminescence immunoassay (CLIA) is used to detect microorganisms for short time and low cost, but the sensitivity of specific identification and detection needs to be improved. The recognition of alternative antibodies and related preprocessing techniques are the focus of future research.
【作者单位】：北京市理化分析测试中心北京市食品安全分析测试工程技术研究中心;北京勤邦生物技术有限公司北京市食品安全免疫快速检测工程技术研究中心;
【基金】：国家重大科学仪器设备开发专项(2013YQ140371) 国家重点研发计划资助(2016YFF0203802) 北京市科技计划项目(Z161100000916001) 北京市自然科学基金项目(2162018)
【分类号】：O657.3;R446.5

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