水通道蛋白亚型3在脓毒症肺血管通透性中的作用及SS-31对其的影响

发布时间：2018-09-14 14:55

【摘要】：目的探讨水通道蛋白亚型3(aquaporin 3,Aqp3)在脓毒症肺血管通透性中的作用及SS-31对其的影响。方法采用SD大鼠盲肠结扎穿孔复制脓毒症模型。在整体动物水平上分为假手术组,脓毒症6、12 h组,在离体细胞水平分为空白对照组,LPS刺激6、12 h组,分别观察脓毒症和脂多糖(lipopolysaccharide,LPS)刺激6、12 h后肺静脉Aqp3的表达和血管通透性的变化,以及干扰Aqp3后内皮细胞通透性的变化。进一步观察抗氧化剂SS-31对Aqp3的表达与血管通透性的作用。结果脓毒症和LPS刺激内皮细胞后Aqp3表达和肺血管通透性均明显增加,与假手术组和空白对照组比较差异有统计学意义(P0.05)。用RNAi抑制Aqp3的表达,可明显拮抗由LPS刺激引起的内皮细胞通透性增加,与LPS刺激组相比差异有统计学意义(P0.05)。在整体动物和离体细胞水平应用抗氧化剂SS-31均可降低由脓毒症和LPS刺激引起的Aqp3表达和血管通透性的增加,与脓毒症和LPS刺激组比较差异有统计学意义(P0.05)。结论 Aqp3参与了脓毒症肺血管通透性的调节,抗氧化措施可降低脓毒症肺血管通透性及下调Aqp3的表达。
[Abstract]:Objective to investigate the role of aquaporin subtype 3 (aquaporin 3) in pulmonary vascular permeability in sepsis and the effect of SS-31 on it. Methods sepsis model was established by cecal ligation and perforation in SD rats. At the whole animal level, they were divided into sham operation group, sepsis 6h 12 h group and in vitro control group. The changes of Aqp3 expression and vascular permeability of pulmonary vein were observed after stimulation of sepsis and lipopolysaccharide (lipopolysaccharide,LPS) for 6h. And the change of endothelial cell permeability after interfering with Aqp3. To investigate the effect of antioxidant SS-31 on the expression of Aqp3 and vascular permeability. Results the expression of Aqp3 and pulmonary vascular permeability were significantly increased after stimulation of endothelial cells by sepsis and LPS, which were significantly different from those in sham operation group and blank control group (P0.05). Inhibition of Aqp3 expression by RNAi could significantly antagonize the increase of endothelial cell permeability induced by LPS stimulation, which was significantly different from that of LPS stimulation group (P0.05). The application of antioxidant SS-31 at the whole animal and in vitro level could decrease the expression of Aqp3 and increase of vascular permeability induced by sepsis and LPS stimulation, which was significantly different from that of sepsis and LPS stimulation group (P0.05). Conclusion Aqp3 is involved in the regulation of pulmonary vascular permeability in sepsis. Antioxidation measures can reduce pulmonary vascular permeability and down-regulate the expression of Aqp3.
【作者单位】：第三军医大学大坪医院野战外科研究所第二研究室创伤、烧伤与复合伤国家重点实验室;
【基金】：国家自然科学基金面上项目(81570441)~~
【分类号】：R459.7

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