鲍曼不动杆菌对8种常用抗菌药物敏感试验不同方法的比较

发布时间：2018-10-16 14:51

【摘要】：目的分析河北省细菌耐药性监测网(Hebarin)鲍曼不动杆菌耐药性监测数据,验证不同药敏试验方法结果,提高耐药性监测数据质量,指导临床实验室使用合理的药敏试验方法。方法回顾性分析Hebarin鲍曼不动杆菌药敏数据。收集126株鲍曼不动杆菌,应用E试验法、纸片扩散法和VITEK仪器法检测其对哌拉西林-他唑巴坦、阿米卡星、庆大霉素、环丙沙星、左氧氟沙星、头孢他啶、亚胺培南和美罗培南的药敏试验结果。结果 126株鲍曼不动杆菌药敏试验结果显示,3种方法中细菌对哌拉西林-他唑巴坦、阿米卡星、庆大霉素、左氧氟沙星、头孢他啶药敏结果存在明显差异(P0.05);纸片扩散法的分类一致性、一般错误率、严重错误率、极严重错误率均在可接受范围,纸片扩散法和VITEK仪器法对哌拉西林-他唑巴坦、阿米卡星、左氧氟沙星的分类一致性存在明显差异(P0.05)。结论不同的药敏试验方法会导致不同的耐药结果,从而使耐药监测数据出现方法学的差异。应用VITEK仪器进行药敏试验时,哌拉西林-他唑巴坦、阿米卡星、左氧氟沙星的药敏结果要结合纸片扩散法或E试验方法进行验证,以便更加准确地指导临床用药。
[Abstract]:Objective to analyze the drug resistance monitoring data of Acinetobacter baumannii in Hebei Province, verify the results of different drug sensitivity test methods, improve the quality of drug resistance monitoring data, and guide the clinical laboratory to use reasonable drug sensitivity test methods. Methods the drug sensitivity data of Acinetobacter baumannii Hebarin were analyzed retrospectively. 126 strains of Acinetobacter baumannii were collected and tested for p-piperacillin tazobactam, amikacin, gentamicin, ciprofloxacin, levofloxacin, ceftazidime by E test, disk diffusion and VITEK. The results of imipenem and meropenem susceptibility tests. Results the drug sensitivity test of 126 strains of Acinetobacter baumannii showed that the three methods were p-piperacillin-tazobactam, amikacin, gentamicin, levofloxacin. The results of ceftazidime drug sensitivity were significantly different (P0.05), the classification consistency, general error rate, serious error rate, extremely serious error rate of disc diffusion method were all acceptable, the disc diffusion method and VITEK instrument method for piperacillin tazobactam, There were significant differences in classification consistency between amikacin and levofloxacin (P0.05). Conclusion different methods of drug sensitivity test may lead to different results of drug resistance, so that there are differences in methodology of drug resistance monitoring data. The drug sensitivity of piperacillin-tazobactam, amikacin and levofloxacin should be verified by disk diffusion or E test in order to guide clinical drug use more accurately.
【作者单位】：承德医学院附属医院检验科;河北医科大学第二医院检验科;河北省临床检验中心;沧州市人民医院检验科;河北医科大学第三医院检验科;
【基金】：河北省科技厅基础条件平台建设项目(河北省医学微生物菌种资源标准平台建设10966142D)
【分类号】：R446.5

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