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不同真空抗凝管保存对血醇含量稳定性的影响

发布时间:2018-10-21 16:56
【摘要】:目前对酒驾取血存在血储容器种类繁多、缺乏规范的现象,为进一步优选血储容器,最大限度保证血醇含量测定结果的稳定,为血样存储最适容器的选择和交管部门制定相应规范、标准提供基础的数据。本实验选取5种常见的真空抗凝管,在恒温冰箱(4~8℃)下存储静脉鲜血,采用GC法测定血液0、3 d、7 d、14 d、21 d和28 d的乙醇动态含量。结果显示:血储容器为枸橼酸钠(1:4)黑色帽管在3 d后、氟化钠-草酸钾灰色帽管在7 d后血醇含量呈不断显著下降的趋势(p0.05);EDTA-k2紫色帽管、枸橼酸钠(1:9)蓝色帽管、肝素绿色帽管在21 d内酒精含量稳定性较好,之后显著下降(p0.05)。5种采血管以EDTA-k2紫色帽管、枸橼酸钠(1:9)蓝色帽管、肝素绿色帽管在21 d内为较佳血储容器,其余2种抗凝管不宜作为血醇检测的血储容器。
[Abstract]:At present, there are many kinds of blood storage containers for drinking driving blood, which is lack of standard. In order to further optimize the selection of blood storage containers and to ensure the stability of the determination results of blood alcohol content to the maximum extent, To provide basic data for the selection of optimal vessel for blood sample storage and for traffic control department. In this experiment, five kinds of common vacuum anticoagulant tubes were used to store venous blood in a constant temperature refrigerator (48 鈩,

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