广州地区伊朗献血人群CD36表型筛查及基因型鉴定
发布时间：2018-11-06 13:58
【摘要】：目的了解广州地区伊朗籍献血者CD36表型及基因型。方法对2016年在广州血液中心捐献机采血小板的53例伊朗人献血者标本,应用流式细胞仪分别检测血小板和单核细胞上CD36抗原的表达。对CD36抗原表达异常者提取DNA,采用PCR-SBT测序做CD36的基因型鉴定并做基因突变分析。结果本组伊朗人群的CD36抗原表达异常率7.55%(4/53),其中2例的CD36抗原在血小板上表达含量低,1例为血小板和单核细胞上均无表达(即CD36Ⅰ型缺失),1例为单核细胞上CD36表达量低,由此得出CD36缺失表达的频率为1.89%(1/53)。PCRSBT法分析:CD36抗原表达异常4例的CD36基因型中,血小板上低表达CD36抗原的2例发生基因突变,分别是exon 10-975T/G和exon11 del CTA,且均为新的基因突变;2例CD36基因型正常。结论本组伊朗献血人群里CD36抗原表达异常者中存在新的基因突变,其对CD36抗原表达的影响尚需进一步认证。
[Abstract]:Objective to investigate the phenotype and genotype of CD36 in Iranian blood donors in Guangzhou. Methods the expression of CD36 antigen on platelet and monocyte were detected by flow cytometry in 53 human blood donors collected from Guangzhou Blood Center in 2016. DNA, was extracted from patients with abnormal CD36 antigen expression. CD36 genotypes were identified by PCR-SBT sequencing and gene mutation was analyzed. Results the abnormal rate of CD36 antigen expression in Iranian population was 7.55% (4 / 53). In 2 cases, the expression of CD36 antigen was low on platelets, and in 1 case, there was no expression of CD36 antigen on platelets and monocytes (i.e. CD36 type 鈪，
本文编号：2314478
[Abstract]:Objective to investigate the phenotype and genotype of CD36 in Iranian blood donors in Guangzhou. Methods the expression of CD36 antigen on platelet and monocyte were detected by flow cytometry in 53 human blood donors collected from Guangzhou Blood Center in 2016. DNA, was extracted from patients with abnormal CD36 antigen expression. CD36 genotypes were identified by PCR-SBT sequencing and gene mutation was analyzed. Results the abnormal rate of CD36 antigen expression in Iranian population was 7.55% (4 / 53). In 2 cases, the expression of CD36 antigen was low on platelets, and in 1 case, there was no expression of CD36 antigen on platelets and monocytes (i.e. CD36 type 鈪，
本文编号：2314478
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