睡眠剥夺对大鼠海马miRNAs的影响

发布时间：2018-11-14 16:38

【摘要】：目的:探讨睡眠剥夺(sleep deprivation,SD)对成年大鼠海马miR132,miR138,miR127,miR128及let-7b的影响。方法:成年雄性Sprague-Dawley30只随机分为对照组(con),睡眠剥夺8 h组(sd8h),睡眠剥夺1 d组(sd1),睡眠剥夺3 d组(sd3),睡眠剥夺6 d组(sd6);采用改良多平台睡眠剥夺法(MMPM)建立大鼠SD模型,SYBRA greenⅠ荧光定量RT-PCR检测大鼠海马miRNAs的变化。结果:RT-PCR结果显示:(1)海马sd8 h组和sd1组miR132水平均低于control组(P0.05),但sd3组高于control组(P0.05);(2)海马sd8 h、sd3组miR138水平均低于control组(P0.01)但sd1组高于control组(P0.01);(3)海马sd8 h、sd1、sd3组miR127水平均低于control组(P0.05),但sd6组高于control组(P0.05);(4)海马sd1组miRl28水平低于control组(P0.05),sd8h组高于control组(P0.05);(5)海马sd8h、sdl、sd3组let-7b水平均高于control组(P0.05)。结论:睡眠剥夺可能与海马的miR132,miR138,miR127,miR128及let-7b的水平有关。
[Abstract]:Objective: to investigate the effects of sleep deprivation (sleep deprivation,SD) on miR132,miR138,miR127,miR128 and let-7b in hippocampus of adult rats. Methods: adult male Sprague-Dawley30 were randomly divided into (con), sleep deprivation 8 h group (sd8h), sleep deprivation 1 day group (sd1), sleep deprivation 3 days group (sd3) and sleep deprivation 6 d group (sd6). The changes of rat hippocampal miRNAs were detected by modified multi-platform sleep deprivation method (MMPM) to establish rat SD model, SYBRA green 鈪，

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