非靶标~1H-NMR尿液代谢组学筛选生物标志物组用于心理亚健康的诊断识别

发布时间：2018-11-21 08:53

【摘要】：心理亚健康(psychological suboptimal health)临床症状表现多样,且无与其密切相关的具体生化指标,因而心理亚健康的判别和诊断成为目前研究中的重点和难点。本研究旨在发现并评价能够用于临床诊断的尿液生物标志物组,为心理亚健康的临床诊断提供依据。通过纳入符合入组标准的24例心理亚健康及18例健康对照受试者,采集空腹晨尿经预处理后进行核磁共振氢谱法(1H-nuclear magnetic resonance spectroscopy,1H-NMR)测试。采用单变量、多元统计及随机森林算法对数据运算分析,以筛选造成组间差异的主要代谢物,发现脯氨酸、苯丙氨酸、葫芦巴碱、酮戊二酸、丙氨酸、柠檬酸、吲哚硫酸酚、乳酸、肌酸、牛磺酸及丙三醇11种代谢物的组间浓度表现出显著性差异(P0.05,0.01),提示可作为候选诊断生物标志物。进一步结合受试者工作曲线(receiver operating characteristic curve,ROC)及曲线下面积(area under the curve,AUC)对其作为诊断生物标志物组的能力进行评价。结果显示,单一生物标志物对心理亚健康的诊断能力较低,而将柠檬酸、葫芦巴碱及丙三醇3种物质联合应用后诊断能力明显提升(AUC=0.8)。上述结果提示,柠檬酸、葫芦巴碱及丙三醇3种候选生物标志物,可作为生物标志物组用于心理亚健康的诊断。生物标志物组的发现及其应用对于提高心理亚健康诊断与识别的准确度具有重要意义。
[Abstract]:The clinical symptoms of mental sub-health (psychological suboptimal health) are various and there are no specific biochemical indexes closely related to it. Therefore, the discrimination and diagnosis of mental sub-health has become the focus and difficulty in the current research. The purpose of this study was to identify and evaluate urine biomarkers for clinical diagnosis, and to provide evidence for clinical diagnosis of mental subhealth. The fasting morning urine samples were collected from 24 subhealthy subjects and 18 healthy controls. The fasting morning urine was pretreated and tested by nuclear magnetic resonance spectroscopy (1H-nuclear magnetic resonance spectroscopy,1H-NMR). Single variable, multivariate statistics and random forest algorithm were used to analyze the data. Proline, phenylalanine, cucurbitine, ketoglutaric acid, alanine, citric acid, indole sulfate were found. The concentrations of lactic acid, creatine, taurine and triglyceride were significantly different among the 11 metabolites (P0.05 / 0.01), suggesting that they could be used as candidate biomarkers for diagnosis. The ability to diagnose biomarkers was further evaluated with (receiver operating characteristic curve,ROC and area under the curve (area under the curve,AUC). The results showed that the diagnostic ability of single biomarker for mental sub-health was lower, but the diagnostic ability of citric acid, cucurbitine and propanol was significantly improved (AUC=0.8) after the combination of citric acid, cucurbitine and propanol. These results suggest that citric acid, cucurbitine and propanetriol can be used as biomarkers for the diagnosis of mental subhealth. The discovery of biomarker group and its application are of great significance to improve the accuracy of mental subhealth diagnosis and recognition.
【作者单位】：山西医科大学第一医院中医科;山西大学中医药现代研究中心;山西大学化学化工学院;
【基金】：山西省科技研究计划(No.201601D011122,201603D321077,201603D3113013) 山西省科技创新重点团队(No.201605D131045-18) 山西省重点实验室(No.201605D111004)~~
【分类号】：R446.12

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