微波消解-石墨炉原子吸收光谱法测定血和尿中的铅和镉

发布时间：2018-11-28 14:34

【摘要】：目的建立微波消解-石墨炉原子吸收光谱法测定血和尿中铅和镉含量的方法,为临床铅镉中毒提供诊疗依据。方法取0.50 ml血液和尿液,在HNO3/H2O2消解剂中微波消解5 min,然后以硫酸铵-磷酸二氢铵混合溶液为基体改进剂,采用石墨炉原子吸收光谱法对消解液中的铅镉含量进行测定。结果铅、镉在分别在0.0~160μg/L和0.0~16.0μg/L范围内线性良好(相关系数分别为铅0.9993,镉0.9987),方法检出限(3 s/b)分别为0.36μg/L和3.24μg/L。加标回收率在97.8%~103.0%之间,测定值的相对标准偏差(n=6)在1.76%~6.81%之间。结论该方法快速灵敏,精密度和准确度均符合要求,可为临床诊断铅镉中毒提供一个简便准确的检测方法。
[Abstract]:Objective to establish a method for determination of lead and cadmium in blood and urine by microwave digestion-graphite furnace atomic absorption spectrometry. Methods the blood and urine of 0. 50 ml were digested by microwave for 5 min, in the HNO3/H2O2 digestion medium. The mixture of ammonium sulfate and ammonium dihydrogen phosphate was used as the matrix modifier. The content of lead and cadmium in digestion solution was determined by graphite furnace atomic absorption spectrometry. Results the linear ranges of lead and cadmium were 0.0160 渭 g / L and 0.016 渭 g / L (correlation coefficients were 0.9993 and 0.9987 respectively), and the detection limits (3 s / b) were 0.36 渭 g / L and 3.24 渭 g / L, respectively. The recoveries were between 97.8% and 103.0%, and the relative standard deviation (nc6) of the determination values was between 1.76% and 6.81%. Conclusion the method is rapid, sensitive, accurate and accurate. It can provide a simple and accurate method for clinical diagnosis of lead cadmium poisoning.
【作者单位】：贵州医科大学法医学院法医司法鉴定中心;贵州省疾病预防控制中心;
【基金】：贵州省科技基金项目(黔科合SY字[2013]3044号)(黔科合J字[2014]2008号) 贵州省高校工程技术研究中心项目(黔科合KY字[2013]114号) 贵州省教育厅产学研基地项目(黔教合KY字[2012]031号) 贵州省发改委工程研究中心项目
【分类号】：O657.31;R446.1

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