不同电压的脉冲射频对大鼠CCI模型痛阈值及神经结构的影响

发布时间：2019-01-01 12:52

【摘要】：目的:观察不同电压的脉冲射频(PRF)对大鼠坐骨神经慢性缩窄损伤(CCI)疼痛模型热痛阈(TWL)、机械痛阈(MWT)及坐骨神经结构的影响。方法:72只成年雄性SD大鼠,随机均分为4组:假射频组、PRF-45V组、PRF-55V组、PRF-65V组,每组18只。CCI模型建立成功后,假射频组仅放置射频针120s而无脉冲干预,其余三组分别行45V、55V与65V电压的PRF干预(频率2Hz、时间120s)。分别在制模前、模型成立(即制模后7 d)、PRF后1、4、7、10和14 d检测各大鼠的TWL值和MWT值的变化。并于PRF后14 d取大鼠右侧PRF干预处坐骨神经干,电镜下观察其结构变化。结果:模型成立后各组大鼠TWL值和MWT值均显著低于制模前,其差异有统计学意义(P0.05);制模前和模型成立时各组大鼠TWL值和MWT值比较,差异无统计学意义(P0.05);与假射频组比较,PRF-45V组、PRF-55V组和PRF-65V组在PRF干预后各时点TWL值和MWT值均较高,差异有统计学意义(P0.05);与PRF-45V组比较,PRF-55V组和PRF-65V组PRF后14 d时TWL值和MWT值均较高,差异有统计学意义(P0.05)。电镜下各组大鼠坐骨神经均无脱髓鞘等严重损伤,PRF-65V组可见大量外形不规则的髓鞘出现,板层结构肿胀变粗,且程度高于PRF-55V组和PRF-45V组。结论:1.CCI大鼠经PRF干预后早期痛阈明显恢复,且高电压55V和65V的痛阈恢复程度高于低电压45V,55V与65V两者间痛阈改变差异不明显。2.本实验所设立电压的PRF对坐骨神经仅造成轻微损伤,PRF-65V组有髓神经纤维水肿程度高于PRF-55V组和PRF-45V组。
[Abstract]:Aim: to observe the effects of pulsed radiofrequency (PRF) at different voltages on the thermal pain threshold (TWL), mechanical pain threshold (MWT) and the structure of sciatic nerve in rats with (CCI) pain induced by chronic constriction injury of sciatic nerve. Methods: 72 adult male SD rats were randomly divided into 4 groups: pseudo-radio frequency group, PRF-45V group, PRF-55V group and PRF-65V group, 18 rats in each group. After the successful establishment of CCI model, the pseudo-radio frequency group was only placed with radio frequency needle for 120 s without pulse intervention. The other three groups were treated with PRF at 45 V, 55 V and 65 V, respectively (frequency 2 Hz, time 120 s). The changes of TWL and MWT values of each rat were detected at 10 and 14 days after modeling (7 d), PRF after modeling). At 14 days after PRF, the sciatic nerve trunk was taken from the right side of PRF of rats, and the structural changes were observed under electron microscope. Results: the values of TWL and MWT in each group were significantly lower than those before modeling (P0.05), but there was no significant difference in TWL and MWT between the groups before model making and when the model was established (P0.05). Compared with the pseudo-radiofrequency group, the TWL and MWT values of PRF-45V group, PRF-55V group and PRF-65V group were higher at each time point after PRF intervention, the difference was statistically significant (P0.05). Compared with PRF-45V group, the TWL value and MWT value of PRF-55V group and PRF-65V group were higher at 14 days after PRF, the difference was statistically significant (P0.05). There was no serious injury of sciatic nerve such as demyelination under electron microscope. A large number of irregular myelin sheath appeared in PRF-65V group, and the lamellar structure was swollen and thickened, and the degree was higher than that in PRF-55V group and PRF-45V group. Conclusion: the early pain threshold of 1.CCI rats recovered obviously after PRF intervention, and the recovery degree of pain threshold of high voltage 55V and 65V was higher than that of low voltage 45V 55V and 65V respectively. 2. The degree of edema of myelinated nerve fibers in PRF-65V group was higher than that in PRF-55V group and PRF-45V group.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R402

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