江苏省南京市和无锡市两地首次检出人星状病毒HAstV-1a基因亚型

发布时间：2019-03-05 09:54

【摘要】：了解江苏省南京市和无锡市两地夏秋季急性胃肠炎人星状病毒(HastV)的基因亚型。收集2015~2016年6~11月江苏省南京市和无锡市两地夏秋季急性胃肠炎患者粪便标本,采用real-time RT-PCR法检测粪便中HAstV的病毒核酸,对阳性标本ORF2区进行RT-PCR扩增及测序,与GenBank公开的序列进行比对,分析基因亚型情况。江苏省南京市和无锡市两地夏秋季急性胃肠炎HAstV感染的比例为15.26%,其中50%为合并其它急性胃肠炎病毒感染,HAstV感染以秋季流行为主,包含HAstV-1a(group 2)亚型和HAstV-4c基因亚型,并且这2个亚型在两地均有分布。HastV在婴幼儿人群中感染率较高;HAstV-1a亚型为在江苏省南京市和无锡市两地首次发现及报道的亚型,可能为欧美输入性毒株,本研究为进一步了解国内HAstV分子流行特征提供依据。
[Abstract]:To investigate the gene subtype of human stellate virus (HastV) of acute gastroenteritis in Nanjing and Wuxi in summer and autumn. The fecal samples of patients with acute gastroenteritis in summer and autumn in Nanjing and Wuxi, Jiangsu Province from June to November, 2015-2016 were collected. The viral nucleic acid of HAstV in feces was detected by real-time RT-PCR, and the ORF2 region of positive samples was amplified by RT-PCR and sequenced. The results were compared with the published sequences of GenBank and analyzed the subtypes of the genes. The HAstV infection rate of acute gastroenteritis in summer and autumn was 15.26% in Nanjing City and Wuxi City, Jiangsu Province, and 50% of them were associated with other acute gastroenteritis virus infection. The prevalence of HAstV infection was mainly in autumn. HastV contains HAstV-1a (group 2) subtype and HAstV-4c gene subtype, and these two subtypes are distributed in both places. HastV infection rate is higher in infants and young children. HAstV-1a subtype was found and reported for the first time in Nanjing and Wuxi, Jiangsu Province, and may be imported from Europe and the United States. This study provides a basis for further understanding the epidemic characteristics of HAstV molecules in China.
【作者单位】：无锡市第五人民医院;南京市妇幼保健院;
【基金】：无锡市卫生计生委重大项目(项目号:z201603);题目:传染病临床生物样本库及大数据分析平台建设~~
【分类号】：R440;R512.5

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